Isolating quality DNA from tissues/cells presents a variety of problems in particular when
plants are used as the source material. The specific characteristics of plants like the …

Background RNA interference (RNAi) is a powerful technique for functional genomics
research in insects. Transgenic plants producing double-stranded RNA (dsRNA) directed …

The phloem sap-sucking hemipteran insect, Aphis craccivora, commonly known as cowpea
aphid, cause major yield loss of important food legume crop chickpea. Among different plant …

Different protocols are usually used for extracting total deoxyribonucleic acid (DNA) from
different plant species of same order and DNA of the associated viruses. Here, we describe …

Type C stay-green mutants are defined as being defective in the pathway of chlorophyll
breakdown, which involves pheophorbide a oxygenase (PAO), required for loss of green …

Current DNA extraction protocols, which require liquid nitrogen, lyophilization and
considerable infrastructure in terms of instrumentation, often impede the application of …

Key message Independent transgenic pigeonpea events were developed using two cry
genes. Transgenic Cry2Aa-pigeonpea was established for the first time. Selected transgenic …

Background Biotrophic interaction between host and pathogen induces generation of
reactive oxygen species that leads to programmed cell death of the host tissue specifically …

Wilt of chickpea caused by Fusarium oxysporum f. sp. ciceris is one of the most severe
diseases of chickpea throughout the world. Variability of pathotypes of F. oxysporum f. sp …

A binary expression vector was constructed containing the insecticidal gene Allium sativum
leaf agglutinin (ASAL), and a selectable nptII marker gene cassette, flanked by lox sites …