The fungal vacuole is an extremely complex organelle that is involved in a wide variety of
functions. The vacuole not only carries out degradative processes, the role most often …

INTRODUCTION Traditionally the proteinases have been regarded as degradative enzymes
which are capa-ble of cleaving proteins into small peptides and amino acids and whose role …

Using a selection for spontaneous mutants that mislocalize a vacuolar carboxypeptidase Y
(CPY)-invertase fusion protein to the cell surface, we identified vacuolar protein targeting …

Under nutrient-deficient conditions, the yeast S. cerevisiae sequesters its own cytoplasmic
components into vacuoles in the form of" autophagic bodies"(Takeshige, K., M. Baba, S …

The Saccharomyces cerevisiae APE1 gene product, aminopeptidase I (API), is a soluble
hydrolase that has been shown to be localized to the vacuole. API lacks a standard signal …

We have constructed a PRC1-SUC2 gene fusion that directs the synthesis in
Saccharomyces cerevisiae of a hybrid polypeptide consisting of a 433-residue amino …

PMR1, a Ca (2+)-adenosine triphosphatase (ATPase) homologue in the yeast
Saccharomyces cerevisiae localizes to a novel Golgi-like organelle. Consistent with a Golgi …

Yeast vacuole protein targeting (vpt) mutants exhibit defects in the sorting and processing of
multiple vacuolar hydrolases. To evaluate the impact these vpt mutations have on the …

The proteinase A structural gene of Saccharomyces cerevisiae was cloned by using an
immunological screening procedure that allows detection of yeast cells which are aberrantly …

Temperature-sensitive secretory mutants (set) of S. cerevisiae have been used to evaluate
the organelles and cellular functions involved in transport of the vacuolar glycoprotein …