Chronos: a cell population dynamics model of CRISPR experiments that improves inference of gene fitness effects
CRISPR loss of function screens are powerful tools to interrogate biology but exhibit a
number of biases and artifacts that can confound the results. Here, we introduce Chronos …
number of biases and artifacts that can confound the results. Here, we introduce Chronos …
Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage
S Riesenberg, N Helmbrecht, P Kanis, T Maricic… - Nature …, 2022 - nature.com
The first step in CRISPR-Cas9-mediated genome editing is the cleavage of target DNA
sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs …
sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs …
Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
CRISPR-Cas9 is a powerful DNA editing tool. A gRNA directs Cas9 to cleave any DNA
sequence with a PAM. However, some gRNA sequences mediate cleavage at higher …
sequence with a PAM. However, some gRNA sequences mediate cleavage at higher …
Molecular and computational strategies to increase the efficiency of CRISPR-based techniques
L Mattiello, M Rütgers, MF Sua-Rojas… - Frontiers in Plant …, 2022 - frontiersin.org
The prokaryote-derived Clustered Regularly Interspaced Palindromic Repeats
(CRISPR)/Cas mediated gene editing tools have revolutionized our ability to precisely …
(CRISPR)/Cas mediated gene editing tools have revolutionized our ability to precisely …
Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs
K Huszár, Z Welker, Z Györgypál, E Tóth… - Nucleic Acids …, 2023 - academic.oup.com
Abstract Streptococcus pyogenes Cas9 (SpCas9) nuclease exhibits considerable position-
dependent sequence preferences. The reason behind these preferences is not well …
dependent sequence preferences. The reason behind these preferences is not well …
[HTML][HTML] Machine learning-based prediction models to guide the selection of Cas9 variants for efficient gene editing
J Li, P Wu, Z Cao, G Huang, Z Lu, J Yan, H Zhang… - Cell Reports, 2024 - cell.com
The increasing emergence of Cas9 variants has attracted broad interest, as these variants
were designed to expand CRISPR applications. New Cas9 variants typically feature higher …
were designed to expand CRISPR applications. New Cas9 variants typically feature higher …
HyCas9-12aGEP: an efficient genome editing platform for Corynebacterium glutamicum
F Zhang, JY Wang, CL Li, WG Zhang - Frontiers in Bioengineering …, 2024 - frontiersin.org
Corynebacterium glutamicum plays a crucial role as a significant industrial producer of
metabolites. Despite the successful development of CRISPR-Cas9 and CRISPR-Cas12a …
metabolites. Despite the successful development of CRISPR-Cas9 and CRISPR-Cas12a …
A meta-analysis of gRNA library screens enables an improved understanding of the impact of gRNA folding and structural stability on CRISPR-Cas9 activity
CRISPR systems are known to be inhibited by unwanted secondary structures that form
within the guide RNA (gRNA). The minimum free energy of predicted secondary structures …
within the guide RNA (gRNA). The minimum free energy of predicted secondary structures …
Sign language recognition of Malayalam alphabets using transfer learning
A Salim - … Conference on Power, Instrumentation, Control and …, 2023 - ieeexplore.ieee.org
Sign languages, like any other natural language, rely on a set of symbols or gestures to
convey meaning. The recognition of these symbols is crucial for the development of …
convey meaning. The recognition of these symbols is crucial for the development of …
An analysis of gRNA sequence dependent cleavage highlights the importance of genomic context on CRISPR-Cas activity
CRISPR-Cas9 is a powerful DNA editing tool. A gRNA directs Cas9 to cleave any DNA
sequence with a PAM. However, some gRNA sequences mediate cleavage at higher …
sequence with a PAM. However, some gRNA sequences mediate cleavage at higher …