[HTML][HTML] Nucleic acid protocols: Extraction and optimization

S El-Ashram, I Al Nasr, X Suo - Biotechnology reports, 2016 - Elsevier
Yield and quality are fundamental features for any researchers during nucleic acid
extraction. Here, we describe a simplified, semi-unified, effective, and toxic material free …

An efficient protocol for isolation of inhibitor-free nucleic acids even from recalcitrant plants

MH Rezadoost, M Kordrostami, HH Kumleh - 3 Biotech, 2016 - Springer
For fast and easy isolation of inhibitor-free genomic DNA even from the toughest plant leaf
samples, including those high in polyphenols and polysaccharides, a protocol has been …

Molecular methods (digital PCR and real‐time PCR) for the quantification of low copy DNA of Phytophthora nicotianae in environmental samples

J Blaya, E Lloret, AB Santísima‐Trinidad… - Pest Management …, 2016 - Wiley Online Library
BACKGROUND Currently, real‐time polymerase chain reaction (qPCR) is the technique
most often used to quantify pathogen presence. Digital PCR (dPCR) is a new technique with …

[PDF][PDF] Application of SSR markers for genetic purity analysis of parental inbred lines and some commercial hybrid maize (Zea mays L.).

Aims: Morphological evaluation of seeds and growing plants used for certification for purity
and variety distinctness in Nigeria is time consuming and expensive. This experiment set to …

Recycling isolation of plant DNA, a novel method

L Zhang, B Wang, L Pan, J Peng - Journal of Genetics and Genomics, 2013 - Elsevier
DNA is one of the most basic and essential genetic materials in the field of molecular
biology. To date, isolation of sufficient and good-quality DNA is still a challenge for many …

Genomic DNA extraction method from mature leaf of lai (Durio kutejensis Becc.)

F Handayani, RA Wulandari… - AGRIVITA Journal of …, 2016 - agrivita.ub.ac.id
Abstract Lai (Durio kutejensis Becc.) is an indigeneous germplasm of Kalimantan which has
some superiorities compared to its close-relative, durian (Durio zibethinus Murr.). Genetic …

[PDF][PDF] Modified method of high quality genomic DNA extraction from mungbean [Vigna radiata (L.) Wilczek] suitable for PCR based amplification

DT Dharajiya, SM Khadia, NK Pagi… - Indian J Sci …, 2017 - researchgate.net
Objectives: Mungbean is one among the foremost necessary pulse crops. It is necessary to
extract good quality genomic DNA for molecular work in plant science. Hence, present study …

Construction of a reference plasmid molecule containing eight targets for the detection of genetically modified crops

X Wang, D Teng, Y Yang, F Tian, Q Guan… - Applied microbiology and …, 2011 - Springer
A standard plasmid containing eight targets was developed for quantitative detection of
genetically modified (GM) soybeans and cotton. These eight targets were joined in tandem …

[PDF][PDF] Metode ekstraksi DNA cabai (Capsicum annuum L.) menggunakan modifikasi buffer CTAB (Cethyl Trimethyl Ammonium Bromide) tanpa nitrogen cair

K Nugroho, RT Terryana, P Lestari - Scripta Biologica, 2017 - core.ac.uk
METODE EKSTRAKSI DNA CABAI (Capsicum annuum L.) MENGGUNAKAN MODIFIKASI
BUFER CTAB (CETHYL TRIMETHYL AMMONIUM BROMIDE) TANPA NITR Page 1 …

[PDF][PDF] Assessment of genetic diversity of maize inbred lines and hybrids in Southern Highlands of Tanzania by using random amplified polymorphic DNA (RAPD) …

BA Mrutu, T Feyissa, J Ndunguru - American Journal of Research …, 2014 - usa-journals.com
Maize is Tanzania's most important food crop, providing 60% of the dietary calories and
more than 50% of utilizable protein. The Southern highlands account for almost 50% of the …