Alternative splicing as a source of phenotypic diversity
A major goal of evolutionary genetics is to understand the genetic processes that give rise to
phenotypic diversity in multicellular organisms. Alternative splicing generates multiple …
phenotypic diversity in multicellular organisms. Alternative splicing generates multiple …
Comprehensive characterization of single-cell full-length isoforms in human and mouse with long-read sequencing
A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read
and long-read (nanopore) sequencing together with a new computational pipeline …
and long-read (nanopore) sequencing together with a new computational pipeline …
From words to complete phrases: insight into single-cell isoforms using short and long reads
The profiling of gene expression patterns to glean biological insights from single cells has
become commonplace over the last few years. However, this approach overlooks the …
become commonplace over the last few years. However, this approach overlooks the …
Identification of astrocyte regulators by nucleic acid cytometry
Multiple sclerosis is a chronic inflammatory disease of the central nervous system.
Astrocytes are heterogeneous glial cells that are resident in the central nervous system and …
Astrocytes are heterogeneous glial cells that are resident in the central nervous system and …
A systematic benchmark of Nanopore long read RNA sequencing for transcript level analysis in human cell lines
The human genome contains more than 200,000 gene isoforms. However, different isoforms
can be highly similar, and with an average length of 1.5 kb remain difficult to study with short …
can be highly similar, and with an average length of 1.5 kb remain difficult to study with short …
Diverse cell-specific patterns of alternative polyadenylation in Drosophila
Most genes in higher eukaryotes express isoforms with distinct 3'untranslated regions
(3'UTRs), generated by alternative polyadenylation (APA). Since 3'UTRs are predominant …
(3'UTRs), generated by alternative polyadenylation (APA). Since 3'UTRs are predominant …
RNA splicing programs define tissue compartments and cell types at single-cell resolution
The extent splicing is regulated at single-cell resolution has remained controversial due to
both available data and methods to interpret it. We apply the SpliZ, a new statistical …
both available data and methods to interpret it. We apply the SpliZ, a new statistical …
BRIE2: computational identification of splicing phenotypes from single-cell transcriptomic experiments
Y Huang, G Sanguinetti - Genome biology, 2021 - Springer
RNA splicing is an important driver of heterogeneity in single cells through the expression of
alternative transcripts and as a determinant of transcriptional kinetics. However, the intrinsic …
alternative transcripts and as a determinant of transcriptional kinetics. However, the intrinsic …
MARVEL: an integrated alternative splicing analysis platform for single-cell RNA sequencing data
WX Wen, AJ Mead, S Thongjuea - Nucleic Acids Research, 2023 - academic.oup.com
Alternative splicing is an important source of heterogeneity underlying gene expression
between individual cells but remains an understudied area due to the paucity of …
between individual cells but remains an understudied area due to the paucity of …
Single cell gene regulatory networks in plants: Opportunities for enhancing climate change stress resilience
RK Tripathi, O Wilkins - Plant, Cell & Environment, 2021 - Wiley Online Library
Global warming poses major challenges for plant survival and agricultural productivity. Thus,
efforts to enhance stress resilience in plants are key strategies for protecting food security …
efforts to enhance stress resilience in plants are key strategies for protecting food security …