A phylogenetic profiling approach identifies novel ciliogenesis genes in Drosophila and C. elegans

J Dobbelaere, TY Su, B Erdi, A Schleiffer… - The EMBO …, 2023 - embopress.org
Cilia are cellular projections that perform sensory and motile functions in eukaryotic cells. A
defining feature of cilia is that they are evolutionarily ancient, yet not universally conserved …

In-cell structural insight into the stability of sperm microtubule doublet

L Tai, G Yin, X Huang, F Sun, Y Zhu - Cell Discovery, 2023 - nature.com
The propulsion for mammalian sperm swimming is generated by flagella beating.
Microtubule doublets (DMTs) along with microtubule inner proteins (MIPs) are essential …

Effect of α-tubulin acetylation on the doublet microtubule structure

SK Yang, S Kubo, CS Black, K Peri, D Dai, T Legal… - Elife, 2024 - elifesciences.org
Acetylation of α-tubulin at the lysine 40 residue (αK40) by αTAT1/MEC-17 acetyltransferase
modulates microtubule properties and occurs in most eukaryotic cells. Previous literatures …

Mechanisms of cilia regeneration in Xenopus multiciliated epithelium in vivo

VG Rao, V Subramanianbalachandar, MM Magaj… - bioRxiv, 2023 - biorxiv.org
Cilia regeneration is a physiological event, and while studied extensively in unicellular
organisms, it remains poorly understood in vertebrates. In this study, using Xenopus …

[HTML][HTML] SUB-Immunogold-SEM reveals nanoscale distribution of submembranous epitopes

KK Miller, P Wang, N Grillet - Research Square, 2024 - ncbi.nlm.nih.gov
Electron microscopy paired with immunogold labeling is the most precise tool for protein
localization. However, these methods are either cumbersome, resulting in small sample …

Ccdc66 regulates primary cilium stability, disassembly and signaling important for epithelial organization

J Deretic, S Cengiz-Emek, E Seyrek, EN Firat-Karalar - bioRxiv, 2024 - biorxiv.org
The primary cilium is a conserved, microtubule-based organelle that transduces signaling
pathways essential for development and homeostasis. It is a dynamic structure that …