A method for the production of cryopreserved aliquots of antigen-preloaded, mature dendritic cells ready for clinical use

B Feuerstein, TG Berger, C Maczek, C Röder… - Journal of …, 2000 - Elsevier
Dendritic cells (DC) are increasingly used as a vaccine. Unfortunately, a satisfactory
cryopreservation of DC in the absence of FCS is not yet available, so that laborious repeated …

Massive immune haemolysis after allogeneic peripheral blood stem cell transplantation with minor ABO incompatibility

CD Bolan, RW Childs, JL Procter… - British journal of …, 2001 - Wiley Online Library
Immune haemolysis as a result of minor ABO incompatibility is an underappreciated
complication of haematopoietic transplantation. The increased lymphoid content of …

The influence of freezing and storage on the characteristics and functions of human mesenchymal stromal cells isolated for clinical use

M Haack-Sorensen, L Bindslev, S Mortensen, T Friis… - …, 2007 - Taylor & Francis
Background Studies have shown that stem cell therapy could be a novel option for
improving neovascularization and cardiac function in patients with ischemic heart disease …

Dendritic cell biology and the application of dendritic cells to immunotherapy of multiple myeloma

R Hájek, AW Butch - Medical Oncology, 2000 - Springer
Dendritic cells (DCs) are extremely efficient antigen-presenting cells that are potent
stimulators of both B and T cell immune responses. Although DCs are normally present in …

Isolation and/or preservation of dendritic cells for prostate cancer immunotherapy

GP Murphy, AL Boynton, BA Tjoa - US Patent 5,788,963, 1998 - Google Patents
Methods and compositions for use of human dendritic cells to activate T cells for
immunotherapeutic responses against primary and metastatic prostate cancer are disclosed …

Improvement of bone marrow mononuclear cells cryopreservation methods to increase the efficiency of dendritic cell production

H Kysielova, K Yampolska, T Dubrava, O Lutsenko… - Cryobiology, 2022 - Elsevier
Cryopreservation is now considered an integral part of the biotechnological process,
exploiting different types of cells and tissues in clinical practice. Among them, dendritic cells …

A cryopreservation method of human peripheral blood mononuclear cells for efficient production of dendritic cells

M Makino, M Baba - Scandinavian journal of immunology, 1997 - Wiley Online Library
The establishment of a cryopreservation method for unstimulated fresh peripheral blood
mononuclear cells (PBMC) with nearly 100% viability would greatly contribute to the conduct …

The influence of cryopreservation on cytokine production by human T lymphocytes

SY Wang, ML Hsu, CH Tzeng, HC Hsu, CK Ho - Cryobiology, 1998 - Elsevier
Human T lymphocytes isolated from peripheral blood were cryopreserved at− 196° C for
different periods of 3, 14, 21, 35, and 50 days. Viability and cytokine-producing activity of T …

Cryopreservation of immature monocyte-derived dendritic cells results in enhanced cell maturation but reduced endocytic activity and efficiency of adenoviral …

J John, J Hutchinson, A Dalgleish, H Pandha - Journal of immunological …, 2003 - Elsevier
To date, phase I/II dendritic cell (DC)-based cancer vaccine trials have required repeated
venesection or leukapheresis to generate the DCs. Previous studies have suggested that …

In vitro evaluation of vascular endothelial and smooth muscle cell survival and apoptosis in response to hypothermia and freezing

KN Tatsutani, JD Joye, R Virmani, MJ Taylor - CryoLetters, 2005 - ingentaconnect.com
The success of endovascular techniques such as balloon angioplasty and stenting in the
treatment of atherosclerotic vascular disease has been limited by an aggressive proliferative …