Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Fluorescence microscopy, with its molecular specificity, is one of the major characterization
methods used in the life sciences to understand complex biological systems. Super …

We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the
emitter with a local intensity minimum of excitation light, MINFLUX minimizes the …

Fluorescence microscopy is a key driver of discoveries in the life sciences, with observable
phenomena being limited by the optics of the microscope, the chemistry of the fluorophores …

Super-resolution microscopy opened diverse new avenues of research by overcoming the
resolution limit imposed by diffraction. Exploitation of the fluorescent emission of individual …

After three decades of developments, single particle tracking (SPT) has become a powerful
tool to interrogate dynamics in a range of materials including live cells and novel catalytic …

Single-molecule super-resolution fluorescence microscopy and single-particle tracking are
two imaging modalities that illuminate the properties of cells and materials on spatial scales …

Optical microscopy has for centuries been a key tool to study living cells with minimum
invasiveness. The advent of single molecule techniques over the past two decades has …

ThunderSTORM is an open-source, interactive and modular plug-in for ImageJ designed for
automated processing, analysis and visualization of data acquired by single-molecule …