The field of metabolic engineering is primarily concerned with improving the biological
production of value-added chemicals, fuels and pharmaceuticals through the design …

Corynebacterium glutamicum, Escherichia coli, and Saccharomyces cerevisiae in particular,
have become established as important industrial workhorses in biotechnology. Recent years …

Respiration in the light (RL) releases CO2 in photosynthesizing leaves and is a
phenomenon that occurs independently from photorespiration. Since RL lowers net carbon …

Background Cis, cis-muconic acid (MA) is a dicarboxylic acid of recognized industrial value.
It provides direct access to adipic acid and terephthalic acid, prominent monomers of …

Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

Metabolic engineering is a new field with applications in the production of chemicals, fuels,
materials, pharmaceuticals, and medicine at the genetic level. The field's novelty is in the …

Recently, there has been an increasing interest in stoichiometric analysis of metabolic flux
distributions. Flux balance methods only require information about metabolic reaction …

Metabolic flux analysis using 13C-labeled substrates has become an important tool in
metabolic engineering. It allows the detailed quantification of all intracellular fluxes in the …

We present here a broad overview of the field of metabolic engineering, describing in the
first section the key fundamental principles that define and distinguish it, as well as the …

In many organisms, metabolite interconversion at the phosphoenolpyruvate (PEP)–pyruvate–
oxaloacetate node involves a structurally entangled set of reactions that interconnects the …