The present article highlights the rationale, potential and flexibility of tumor spheroid mono-
and cocultures for implementation into state of the art anti-cancer therapy test platforms …

Multicellular tumor spheroid models (MCTS) are often coined as 3D in vitro models that can
mimic the microenvironment of tissues. MCTS have gained increasing interest in the nano …

Although used in academic research for several decades, 3D culture models have long
been regarded expensive, cumbersome and unnecessary in drug development processes …

Over the past few years, establishment and adaptation of cell-based assays for drug
development and testing has become an important topic in high-throughput screening …

The growth of tumour cells as three‐dimensional multicellular spheroids in vitro has led to
important insights in tumour biology, since properties of the in vivo‐tumour such as …

Four rat embryo fibroblast (REF) cell lines with defined oncogenic transformation were used
to study the relationship between tumorigenic conversion, metabolism, and development of …

Multicellular spheroids composed of transformed cells are known to mimic the growth
characteristics of tumors and to develop gradients in proliferation with increasing size. This …

Cells in the inner region of multicellular spheroids markedly reduce their oxygen
consumption rate, presumably in response to their stressful microenvironment. To determine …

Recent advances in biotechnology have allowed the production of new types of
macromolecular therapeutic agents (antibodies, immunotoxins, cytokines, extracellular …

Multicellular tumor spheroids have proven to be valuable models of the interactions between
the extracellular microenvironment and the metabolism, proliferation and viability of tumor …