Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

From its start as a small‐scale in vitro system to study fundamental translation processes,
cell‐free protein synthesis quickly rose to become a potent platform for the high‐yield …

Malaria is among the most serious infectious diseases affecting humans, accounting for
approximately half a million deaths each year. Plasmodium falciparum causes most life …

Cell-free protein expression plays an important role in biochemical research. However, only
recent developments led to new methods to rapidly synthesize preparative amounts of …

Biopharmaceutical products are of great importance in the treatment or prevention of many
diseases and represent a growing share of the global pharmaceutical market. The usual …

Highlights•Cell-free expression is a highly multidisciplinary bioengineering technology.•Cell-
free expression is fast, quantitative and a safe synthetic biology environment.•TXTL …

The continued global burden of malaria can in part be attributed to a complex lifecycle, with
both human hosts and mosquito vectors serving as transmission reservoirs. In preclinical …

The study of antigenic targets of naturally-acquired immunity is essential to identify and
prioritize antigens for further functional characterization. We measured total IgG antibodies …

Highlights•PCR products can be used to make proteins quickly and easily in a cell-free
system.•Recent advances have widened the application space of such cell-free …

Cell-free protein synthesis (CFPS) systems are gaining more importance as universal tools
for basic research, applied sciences, and product development with new technologies …