The advancement of super-resolution imaging (SRI) relies on fluorescent proteins with novel
photochromic properties. Using light, the reversibly switchable fluorescent proteins (RSFPs) …

Reversibly switchable fluorescent proteins (RSFPs) serve as markers in advanced
fluorescence imaging. Photoswitching from a non-fluorescent off-state to a fluorescent on …

Proteins of the green fluorescent protein (GFP) family are indispensable for fluorescence
imaging experiments in the life sciences, particularly of living specimens. Their essential role …

Fluorescent proteins have become routine tools for biological imaging. However, their
nanosecond lifetimes on the excited state present computational hurdles to a full …

Photochromic fluorescent proteins play key roles in super-resolution microscopy and
optogenetics. The light-driven structural changes that modulate the fluorescence involve …

We recently developed an algorithm to compute response properties for the state-averaged
complete active space self-consistent field method (SA-CASSCF) that capitalized on sparsity …

RsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolved optical
microscopies, which can be toggled between a fluorescent On state and a nonfluorescent …

Neutron crystallography was used to directly locate two protons before and after a pH‐
induced two‐proton transfer between catalytic aspartic acid residues and the hydroxy group …

Reversibly photoswitchable fluorescent proteins find growing applications in cell biology, yet
mechanistic details, in particular on the ultrafast photochemical time scale, remain unknown …

Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program
optoacoustic signal time courses that were temporally unmixed to increase the proteins' …