Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …
[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …
An efficient CRISPR–Cas12a promoter editing system for crop improvement
J Zhou, G Liu, Y Zhao, R Zhang, X Tang, L Li, X Jia… - Nature Plants, 2023 - nature.com
Promoter editing represents an innovative approach to introduce quantitative trait variation
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …
(QTV) in crops. However, an efficient promoter editing system for QTV needs to be …
[HTML][HTML] Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation
Citrus canker caused by Xanthomonas citri subsp. citri (Xcc) is a destructive citrus disease
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …
worldwide. Generating disease-resistant cultivars is the most effective, environmentally …
Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy
Transgene-free plant genome editing in the T0 generation is highly desirable but
challenging,. Here we achieved such a goal using a co-editing strategy via Agrobacterium …
challenging,. Here we achieved such a goal using a co-editing strategy via Agrobacterium …
[HTML][HTML] Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system
Z Zhong, G Liu, Z Tang, S Xiang, L Yang… - Nature …, 2023 - nature.com
Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9),
sourced from a human pathogen, is the most widely used. Here, through in silico data …
sourced from a human pathogen, is the most widely used. Here, through in silico data …
[HTML][HTML] Programmable RNA detection with CRISPR-Cas12a
SR Rananaware, EK Vesco, GM Shoemaker… - Nature …, 2023 - nature.com
Cas12a, a CRISPR-associated protein complex, has an inherent ability to cleave DNA
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …
substrates and is utilized in diagnostic tools to identify DNA molecules. We demonstrate that …
[HTML][HTML] CRISPR/Cas genome editing technologies for plant improvement against biotic and abiotic stresses: advances, limitations, and future perspectives
Y Wang, N Zafar, Q Ali, H Manghwar, G Wang, L Yu… - Cells, 2022 - mdpi.com
Crossbreeding, mutation breeding, and traditional transgenic breeding take much time to
improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game …
improve desirable characters/traits. CRISPR/Cas-mediated genome editing (GE) is a game …
Beyond knockouts: fine‐tuning regulation of gene expression in plants with CRISPR‐Cas‐based promoter editing
X Tang, Y Zhang - New Phytologist, 2023 - Wiley Online Library
The CRISPR‐Cas‐based genome editing field in plants is expanding rapidly. Editing plant
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …
promoters to obtain cis‐regulatory alleles with altered expression levels or patterns of target …