Proteins are the main targets of most drugs; however, system-wide methods to monitor
protein activity and function are still underused in drug discovery. Novel biochemical …

Protein subcellular localization is tightly controlled and intimately linked to protein function in
health and disease. Capturing the spatial proteome—that is, the localizations of proteins and …

Centrioles are conserved microtubule-based organelles that form the core of the centrosome
and act as templates for the formation of cilia and flagella. Centrioles have important roles in …

Over the last few decades, mass spectrometry‐based proteomics has become an
increasingly powerful tool that is now able to routinely detect and quantify thousands of …

Protein quantification without isotopic labels has been a long-standing interest in the
proteomics field. However, accurate and robust proteome-wide quantification with label-free …

Subcellular localization critically influences protein function, and cells control protein
localization to regulate biological processes. We have developed and applied Dynamic …

A better understanding of proteostasis in health and disease requires robust methods to
determine protein half-lives. Here we improve the precision and accuracy of peptide ion …

The centrosome is the primary microtubule organizing center of the cells and templates the
formation of cilia, thereby operating at a nexus of critical cellular functions. Here, we use …

It has become clear that the role of centrosomes extends well beyond that of important
microtubule organizers. There is increasing evidence that they also function as coordination …

During nutrient stress, macroautophagy degrades cellular macromolecules, thereby
providing biosynthetic building blocks while simultaneously remodelling the proteome …