Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Assembly-line polyketide synthases (PKSs) are among the most complex protein
machineries known in nature, responsible for the biosynthesis of numerous compounds …

Bacterial genomes encode the biosynthetic potential to produce hundreds of thousands of
complex molecules with diverse applications, from medicine to agriculture and materials …

C–H functionalization is a very active research field that has attracted the interest of
scientists from many disciplines. This Outlook describes the collaborative efforts within the …

Covering: mid 1990s to 2018 Over the last two decades, diverse approaches have been
explored to generate new polyketides by engineering polyketide synthases (PKSs) …

Macrolides are a large group of natural products that display broad and potent biological
activities and are biosynthesized by type I polyketide synthases (PKSs) and associated …

Next to plants, bacteria account for most of the biomass on Earth. They are found
everywhere, although certain species thrive only in specific ecological niches. These …

Highlights•Many clinical antibiotics are natural products produced by thiotemplate-based
assembly line biosynthetic pathways.•Assembly line pathways provide an opportunity for …

Erythromycin, avermectin and rapamycin are clinically useful polyketide natural products
produced on modular polyketide synthase multienzymes by an assembly-line process in …

Polyketide retrobiosynthesis, where the biosynthetic pathway of a given polyketide can be
reversibly engineered due to the colinearity of the polyketide synthase (PKS) structure and …