Fundamental Aspects of Phase-Separated Biomolecular Condensates

HX Zhou, D Kota, S Qin, R Prasad - Chemical Reviews, 2024 - ACS Publications
Biomolecular condensates, formed through phase separation, are upending our
understanding in much of molecular, cell, and developmental biology. There is an urgent …

Sequence-dependent material properties of biomolecular condensates and their relation to dilute phase conformations

D Sundaravadivelu Devarajan, J Wang… - Nature …, 2024 - nature.com
Material properties of phase-separated biomolecular condensates, enriched with disordered
proteins, dictate many cellular functions. Contrary to the progress made in understanding the …

[HTML][HTML] Sequence-dependent material properties of biomolecular condensates and their relation to dilute phase conformations

DS Devarajan, J Wang, B Szała-Mendyk… - Nature …, 2024 - ncbi.nlm.nih.gov
Material properties of phase-separated biomolecular condensates, enriched with disordered
proteins, dictate many cellular functions. Contrary to the progress made in understanding the …

Protamine-Mediated Tangles Produce Extreme Deoxyribonucleic Acid Compaction

V Ahlawat, A Dhiman, HE Mudiyanselage… - Journal of the …, 2024 - ACS Publications
In sperm cells, protamine replaces histones to compact DNA 10–20 times more than in
somatic cells. To characterize the extreme compaction, we employed confocal microscopy …

[HTML][HTML] The mitochondria chronicles of melatonin and ATP: Guardians of phase separation

D Loh, RJ Reiter - Mitochondrial Communications, 2024 - Elsevier
Phase separation is a thermodynamic process used by all living organisms since the origin
of life to rapidly assemble and disassemble membraneless condensates in response to …

[HTML][HTML] Amino acid-dependent phase equilibrium and material properties of tetrapeptide condensates

Y Zhang, R Prasad, S Su, D Lee, HX Zhou - Cell Reports Physical Science, 2024 - cell.com
The rules of how amino acids dictate the physical properties of biomolecular condensates
are still incomplete. Here, we study condensates formed by tetrapeptides of the form …

Direct and indirect salt effects on homotypic phase separation

M MacAinsh, S Dey, HX Zhou - eLife, 2024 - elifesciences.org
The low-complexity domain of hnRNPA1 (A1-LCD) phase separates in a salt-dependent
manner. Unlike many intrinsically disordered proteins (IDPs) whose phase separation is …

Reentrant Condensation of Polyelectrolytes Induced by Diluted Multivalent Salts: The Role of Electrostatic Gluonic Effects

H Yong - Biomacromolecules, 2024 - ACS Publications
We explore the reentrant condensation of polyelectrolytes triggered by multivalent salts,
whose phase-transition mechanism remains under debate. We propose a theory to study the …

[HTML][HTML] Amino Acid-Dependent Material Properties of Tetrapeptide Condensates

Y Zhang, R Prasad, S Su, D Lee, HX Zhou - bioRxiv, 2024 - ncbi.nlm.nih.gov
Condensates formed by intrinsically disordered proteins mediate a myriad of cellular
processes and are linked to pathological conditions including neurodegeneration. Rules of …

ATP promotes protein coacervation through conformational compaction

Y Zhu, S Lin, L Meng, M Sun, M Liu, J Li… - Journal of Molecular …, 2024 - academic.oup.com
ATP has been recognized as a hydrotrope in the phase separation process of intrinsically
disordered proteins (IDPs). Surprisingly, when using the disordered RG/RGG-rich motif from …