Directed evolution: methodologies and applications

Y Wang, P Xue, M Cao, T Yu, ST Lane… - Chemical reviews, 2021 - ACS Publications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

DNA synthesis technologies to close the gene writing gap

A Hoose, R Vellacott, M Storch, PS Freemont… - Nature Reviews …, 2023 - nature.com
Synthetic DNA is of increasing demand across many sectors of research and commercial
activities. Engineering biology, therapy, data storage and nanotechnology are set for rapid …

Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system

Y Jiang, B Chen, C Duan, B Sun, J Yang… - Applied and …, 2015 - Am Soc Microbiol
An efficient genome-scale editing tool is required for construction of industrially useful
microbes. We describe a targeted, continual multigene editing strategy that was applied to …

Fourth generation biofuel from genetically modified algal biomass: Challenges and future directions

H Shokravi, Z Shokravi, M Heidarrezaei, HC Ong… - Chemosphere, 2021 - Elsevier
Genetic engineering applications in the field of biofuel are rapidly expanding due to their
potential to boost biomass productivity while lowering its cost and enhancing its quality …

Synthetic biology to access and expand nature's chemical diversity

MJ Smanski, H Zhou, J Claesen, B Shen… - Nature Reviews …, 2016 - nature.com
Bacterial genomes encode the biosynthetic potential to produce hundreds of thousands of
complex molecules with diverse applications, from medicine to agriculture and materials …

Systems strategies for developing industrial microbial strains

SY Lee, HU Kim - Nature biotechnology, 2015 - nature.com
Industrial strain development requires system-wide engineering and optimization of cellular
metabolism while considering industrially relevant fermentation and recovery processes. It …

Synthetic DNA synthesis and assembly: putting the synthetic in synthetic biology

RA Hughes, AD Ellington - Cold Spring Harbor …, 2017 - cshperspectives.cshlp.org
The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes,
circuits, and even entire genomes by gene synthesis methods has become an enabling …

Large-scale de novo DNA synthesis: technologies and applications

S Kosuri, GM Church - Nature methods, 2014 - nature.com
For over 60 years, the synthetic production of new DNA sequences has helped researchers
understand and engineer biology. Here we summarize methods and caveats for the de novo …

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

AD Garst, MC Bassalo, G Pines, SA Lynch… - Nature …, 2017 - nature.com
Improvements in DNA synthesis and sequencing have underpinned comprehensive
assessment of gene function in bacteria and eukaryotes. Genome-wide analyses require …

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

J Lian, M HamediRad, S Hu, H Zhao - Nature communications, 2017 - nature.com
Designing an optimal microbial cell factory often requires overexpression, knock-down, and
knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is …