[HTML][HTML] A systematic approach to quantitative Western blot analysis
L Pillai-Kastoori, AR Schutz-Geschwender… - Analytical …, 2020 - Elsevier
Attaining true quantitative data from WB requires that all the players involved in the
procedure are quality controlled including the user. Appropriate protein extraction method …
procedure are quality controlled including the user. Appropriate protein extraction method …
Folding and misfolding of human membrane proteins in health and disease: from single molecules to cellular proteostasis
Advances over the past 25 years have revealed much about how the structural properties of
membranes and associated proteins are linked to the thermodynamics and kinetics of …
membranes and associated proteins are linked to the thermodynamics and kinetics of …
Plant lipids: Key players of plasma membrane organization and function
AM Cassim, P Gouguet, J Gronnier, N Laurent… - Progress in Lipid …, 2019 - Elsevier
The plasma membrane (PM) is the biological membrane that separates the interior of all
cells from the outside. The PM is constituted of a huge diversity of proteins and lipids. In this …
cells from the outside. The PM is constituted of a huge diversity of proteins and lipids. In this …
Lipid nanodiscs for high-resolution NMR studies of membrane proteins
Membrane proteins (MPs) play essential roles in numerous cellular processes. Because
around 70% of the currently marketed drugs target MPs, a detailed understanding of their …
around 70% of the currently marketed drugs target MPs, a detailed understanding of their …
19F NMR as a tool in chemical biology
We previously reviewed the use of 19 F NMR in the broad field of chemical biology [Cobb,
SL; Murphy, CD J. Fluorine Chem. 2009, 130, 132–140] and present here a summary of the …
SL; Murphy, CD J. Fluorine Chem. 2009, 130, 132–140] and present here a summary of the …
Detergent-free isolation of membrane proteins and strategies to study them in a near-native membrane environment
B Krishnarjuna, A Ramamoorthy - Biomolecules, 2022 - mdpi.com
Atomic-resolution structural studies of membrane-associated proteins and peptides in a
membrane environment are important to fully understand their biological function and the …
membrane environment are important to fully understand their biological function and the …
Diblock copolymers enhance folding of a mechanosensitive membrane protein during cell-free expression
The expression and integration of membrane proteins into vesicle membranes is a critical
step in the design of cell-mimetic biosensors, bioreactors, and artificial cells. While …
step in the design of cell-mimetic biosensors, bioreactors, and artificial cells. While …
[HTML][HTML] Apelinergic system structure and function
Apelin and apela (ELABELA/ELA/Toddler) are two peptide ligands for a class A G-protein-
coupled receptor named the apelin receptor (AR/APJ/APLNR). Ligand-AR interactions have …
coupled receptor named the apelin receptor (AR/APJ/APLNR). Ligand-AR interactions have …
Enhancing the stability and homogeneity of non-ionic polymer nanodiscs by tuning electrostatic interactions
The nanodisc technology is increasingly used for structural studies on membrane proteins
and drug delivery. The development of synthetic polymer nanodiscs and the recent …
and drug delivery. The development of synthetic polymer nanodiscs and the recent …
Tailoring the evolution of BL21 (DE3) uncovers a key role for RNA stability in gene expression toxicity
SAH Heyde, MHH Nørholm - Communications Biology, 2021 - nature.com
Gene expression toxicity is an important biological phenomenon and a major bottleneck in
biotechnology. Escherichia coli BL21 (DE3) is the most popular choice for recombinant …
biotechnology. Escherichia coli BL21 (DE3) is the most popular choice for recombinant …