Macromolecular modeling and design in Rosetta: recent methods and frameworks
The Rosetta software for macromolecular modeling, docking and design is extensively used
in laboratories worldwide. During two decades of development by a community of …
in laboratories worldwide. During two decades of development by a community of …
Analysis of a SARS-CoV-2-infected individual reveals development of potent neutralizing antibodies with limited somatic mutation
Antibody responses develop following SARS-CoV-2 infection, but little is known about their
epitope specificities, clonality, binding affinities, epitopes, and neutralizing activity. We …
epitope specificities, clonality, binding affinities, epitopes, and neutralizing activity. We …
Protein design and variant prediction using autoregressive generative models
JE Shin, AJ Riesselman, AW Kollasch… - Nature …, 2021 - nature.com
The ability to design functional sequences and predict effects of variation is central to protein
engineering and biotherapeutics. State-of-art computational methods rely on models that …
engineering and biotherapeutics. State-of-art computational methods rely on models that …
Vaccination induces broadly neutralizing antibody precursors to HIV gp41
A key barrier to the development of vaccines that induce broadly neutralizing antibodies
(bnAbs) against human immunodeficiency virus (HIV) and other viruses of high antigenic …
(bnAbs) against human immunodeficiency virus (HIV) and other viruses of high antigenic …
Germline-encoded amino acid–binding motifs drive immunodominant public antibody responses
EL Shrock, RT Timms, T Kula, EL Mena, AP West Jr… - Science, 2023 - science.org
Despite the vast diversity of the antibody repertoire, infected individuals often mount
antibody responses to precisely the same epitopes within antigens. The immunological …
antibody responses to precisely the same epitopes within antigens. The immunological …
Five computational developability guidelines for therapeutic antibody profiling
MIJ Raybould, C Marks, K Krawczyk… - Proceedings of the …, 2019 - National Acad Sciences
Therapeutic mAbs must not only bind to their target but must also be free from
“developability issues” such as poor stability or high levels of aggregation. While small …
“developability issues” such as poor stability or high levels of aggregation. While small …
[HTML][HTML] Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients' B cells
The COVID-19 pandemic urgently needs therapeutic and prophylactic interventions. Here,
we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput …
we report the rapid identification of SARS-CoV-2-neutralizing antibodies by high-throughput …
Considerations for the design of antibody-based therapeutics
Antibody-based proteins have become an important class of biologic therapeutics, due in
large part to the stability, specificity, and adaptability of the antibody framework. Indeed …
large part to the stability, specificity, and adaptability of the antibody framework. Indeed …
Clonal structure, stability and dynamics of human memory B cells and circulating plasmablasts
GE Phad, D Pinto, M Foglierini, M Akhmedov… - Nature …, 2022 - nature.com
Memory B cells persist for a lifetime and rapidly differentiate into antibody-producing
plasmablasts and plasma cells upon antigen re-encounter. The clonal relationship and …
plasmablasts and plasma cells upon antigen re-encounter. The clonal relationship and …