Human TopBP1 contains nine BRCT domains and functions in DNA replication initiation,
checkpoint signalling, DNA repair and influences transcriptional control. TopBP1 and its …

Assembly of the CMG (CDC-45–MCM-2-7–GINS) helicase is the key regulated step during
eukaryotic DNA replication initiation. Until now, it was unclear whether metazoa require …

A fundamental requirement for all organisms is the faithful duplication and transmission of
the genetic material. Failure to accurately copy and segregate the genome during cell …

Biomolecular polyelectrolyte complexes can be formed between oppositely charged
intrinsically disordered regions (IDRs) of proteins or between IDRs and nucleic acids. Highly …

Human RECQL4, a member of the RecQ helicase family, plays a role in maintaining
genomic stability, but its precise function remains unclear. The N-terminus of RECQL4 has …

Human RecQL4 belongs to the ubiquitous RecQ helicase family. Its N-terminal region
represents the only homologue of the essential DNA replication initiation factor Sld2 of …

Activation of the replicative Mcm2-7 helicase by loading GINS and Cdc45 is crucial for
replication origin firing, and as such for faithful genetic inheritance. Our biochemical and …

Metazoan genomes are copied bidirectionally from thousands of replication origins.
Replication initiation entails the assembly and activation of two CMG helicases (Cdc45⋅ …

Rothmund-Thomson syndrome (RTS) is an autosomal recessive disorder characterized by
defects in the skeletal system, such as bone hypoplasia, short stature, low bone mass, and …

Regulation of cell cycle duration is critical during development, yet the underlying molecular
mechanisms are still poorly understood. The two-cell stage Caenorhabditis elegans embryo …