New insights into tissue culture plant-regeneration mechanisms

Y Long, Y Yang, G Pan, Y Shen - Frontiers in plant science, 2022 - frontiersin.org
Plant regeneration occurs when plants repair or replace damaged structures based on the
totipotency and pluripotency of their cells. Tissue culture is one of the most widely used …

The diverse roles of cytokinins in regulating leaf development

W Wu, K Du, X Kang, H Wei - Horticulture Research, 2021 - academic.oup.com
Leaves provide energy for plants, and consequently for animals, through photosynthesis.
Despite their important functions, plant leaf developmental processes and their underlying …

Pluripotency acquisition in the middle cell layer of callus is required for organ regeneration

N Zhai, L Xu - Nature Plants, 2021 - nature.com
In plant tissue culture, callus forms from detached explants in response to a high-auxin-to-
low-cytokinin ratio on callus-inducing medium. Callus is a group of pluripotent cells because …

A single-cell analysis of the Arabidopsis vegetative shoot apex

TQ Zhang, Y Chen, JW Wang - Developmental Cell, 2021 - cell.com
The shoot apical meristem allows for reiterative formation of new aerial structures throughout
the life cycle of a plant. We use single-cell RNA sequencing to define the cellular taxonomy …

Spatial transcriptomics reveals light-induced chlorenchyma cells involved in promoting shoot regeneration in tomato callus

X Song, P Guo, K Xia, M Wang, Y Liu… - Proceedings of the …, 2023 - National Acad Sciences
Callus is a reprogrammed cell mass involved in plant regeneration and gene transformation
in crop engineering. Pluripotent callus cells develop into fertile shoots through shoot …

Cytokinin signaling in plant development

JJ Kieber, GE Schaller - Development, 2018 - journals.biologists.com
The phytohormone cytokinin plays diverse roles in plant development, influencing many
agriculturally important processes, including growth, nutrient responses and the response to …

Single-cell transcriptome atlas and chromatin accessibility landscape reveal differentiation trajectories in the rice root

TQ Zhang, Y Chen, Y Liu, WH Lin, JW Wang - Nature communications, 2021 - nature.com
Root development relies on the establishment of meristematic tissues that give rise to
distinct cell types that differentiate across defined temporal and spatial gradients. Dissection …

Recent advances in crop transformation technologies

Z Chen, JM Debernardi, J Dubcovsky, A Gallavotti - Nature plants, 2022 - nature.com
Agriculture is experiencing a technological inflection point in its history, while also facing
unprecedented challenges posed by human population growth and global climate changes …

Cytokinin action in response to abiotic and biotic stresses in plants

A Cortleven, JE Leuendorf, M Frank… - Plant, Cell & …, 2019 - Wiley Online Library
The phytohormone cytokinin was originally discovered as a regulator of cell division. Later, it
was described to be involved in regulating numerous processes in plant growth and …

A single-cell RNA sequencing profiles the developmental landscape of Arabidopsis root

TQ Zhang, ZG Xu, GD Shang, JW Wang - Molecular plant, 2019 - cell.com
Cells of eukaryotic multicellular organisms have inherent heterogeneity. Recent advances in
single-cell gene expression studies enable us to explore transcriptional regulation in …