Current approaches in identification and isolation of cancer stem cells

M Akbarzadeh, NF Maroufi… - Journal of cellular …, 2019 - Wiley Online Library
Cancer stem cells (CSCs) are tumor cells with initiating ability, self‐renewal potential, and
intrinsic resistance to conventional therapeutics. Efficient isolation and characterization of …

Research progress on cardiac tissue construction of mesenchymal stem cells for myocardial infarction

GD Yang, DS Ma, CY Ma, Y Bai - Current Stem Cell Research & …, 2024 - ingentaconnect.com
Heart failure is still the main complication affecting the prognosis of acute myocardial
infarction (AMI), and mesenchymal stem cells (MSCs) are an effective treatment to replace …

Density gradient centrifugation compromises bone marrow mononuclear cell yield

C Pösel, K Möller, W Fröhlich, I Schulz, J Boltze… - PloS one, 2012 - journals.plos.org
Bone marrow mononuclear cells (BMNCs) are widely used in regenerative medicine, but
recent data suggests that the isolation of BMNCs by commonly used Ficoll-Paque density …

A novel technique for accelerated culture of murine mesenchymal stem cells that allows for sustained multipotency

CM Caroti, H Ahn, HF Salazar, G Joseph, SB Sankar… - Scientific reports, 2017 - nature.com
Bone marrow derived mesenchymal stem cells (MSCs) are regularly utilized for translational
therapeutic strategies including cell therapy, tissue engineering, and regenerative medicine …

Stem Leydig cell differentiation: gene expression during development of the adult rat population of Leydig cells

EL Stanley, DS Johnston, J Fan… - Biology of …, 2011 - academic.oup.com
Leydig cells are the testosterone-producing cells in the adult male. Adult Leydig cells (ALCs)
develop from stem Leydig cells (SLCs) through at least two intermediate cells, progenitor …

A simple and reliable protocol for long-term culture of murine bone marrow stromal (mesenchymal) stem cells that retained their in vitro and in vivo stemness in long …

BM Abdallah, AM Alzahrani, AM Abdel-Moneim… - Biological Procedures …, 2019 - Springer
Background Bone marrow derived stromal stem cells (BMSCs) are a clonogenic cell
population that is characterized by self-renewal capacity and differentiation potential into …

Temporal microRNA expression during in vitro myogenic progenitor cell proliferation and differentiation: regulation of proliferation by miR-682

Y Chen, J Gelfond, LM McManus… - Physiological …, 2011 - journals.physiology.org
MicroRNAs (miRNAs) regulate gene expression by repressing target genes at the
posttranscriptional level. Since miRNAs have unique expression profiles in different tissues …

不同组织来源间充质干细胞向血管内皮细胞诱导分化的研究与进展

王俊力, 许慧芳, 冯莉, 魏丹, 陈国华 - 中国组织工程研究, 2016 - cjter.com
摘要背景: 间充质干细胞可诱导分化为血管内皮细胞, 在体外可形成血管移植物用于临床治疗缺
血性疾病, 血管组织工程及再生医学等领域. 目的: 总结间充质干细胞的生物学特征 …

间充质干细胞分离方法的研究与进展

蔡金宏, 林春博, 杨渊 - 中国组织工程研究, 2014 - cjter.com
背景: 间充质干细胞作为种子细胞广泛应用于组织工程和临床治疗, 而安全,
高效的分选方法是间充质干细胞建系的前提. 目的: 综述近年来间充质干细胞分选方法的研究 …

Efficient enrichment of hepatic cancer stem-like cells from a primary rat HCC model via a density gradient centrifugation-centered method

W Liu, X Wang, N You, K Tao, T Wang, L Tang, K Dou - PloS one, 2012 - journals.plos.org
Background Because few definitive markers are available for hepatic cancer stem cells
(HCSCs), based on physical rather than immunochemical properties, we applied a novel …