Communities of microorganisms (microbiota) are present in all habitats on Earth and are
relevant for agriculture, health, and climate. Deciphering the mechanisms that determine …

The critical role of the intestinal microbiota in human health and disease is well recognized.
Nevertheless, there are still large gaps in our understanding of the functions and …

With rapid advances in the development of metabolic pathways and synthetic biology
toolkits, a persisting challenge in microbial bioproduction is how to optimally rewire …

CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) have become
ubiquitous approaches to control gene expression in bacteria due to their simple design and …

Several vaccines targeting bacterial pathogens show reduced efficacy upon concurrent viral
infection, indicating that a new vaccinology approach is required. To identify antigens for the …

Competence development in the human pathogen Streptococcus pneumoniae controls
several features such as genetic transformation, biofilm formation, and virulence. Competent …

Highlights•Phage-host interactions have mostly been studied one-by-one using few old
models.•Systematic approaches reveal new molecular mechanisms of phage-host …

Deciphering gene function is fundamental to engineering of microbiology. The clustered
regularly interspaced short palindromic repeats (CRISPR) system has been adapted for …

While genome-wide transposon mutagenesis screens have identified numerous essential
genes in the significant human pathogen Streptococcus pyogenes (group A Streptococcus …

The RNA-guided Cas9s serve as powerful tools for programmable gene editing and
regulation; their targeting scopes and efficacies, however, are always constrained by the …