Cryogenic electron microscopy (cryo-EM) enables structure determination of
macromolecular objects and their assemblies. Although the techniques have been …

Cryo-electron microscopy (cryo-EM) of single-particle specimens is used to determine the
structure of proteins and macromolecular complexes without the need for crystals. Recent …

The HIV-1 envelope glycoprotein (Env) trimer contains the receptor binding sites and
membrane fusion machinery that introduce the viral genome into the host cell. As the only …

Introduction to Proteins provides a comprehensive and state-of-the-art introduction to the
structure, function, and motion of proteins for students, faculty, and researchers at all levels …

Colloidal nanoparticles doped with lanthanide ions can upconvert near-infrared light to
visible frequencies, enabling the application of such materials to biological imaging and …

To fully understand biological processes from the metabolism of a bacterium to the operation
of a human brain, it is necessary to know the three-dimensional (3D) spatial arrangement …

A recent technological breakthrough in electron cryomicroscopy (cryoEM) is the
development of direct electron detection cameras for data acquisition. By bypassing the …

Binding of the gp120 envelope (Env) glycoprotein to the CD4 receptor is the first step in the
HIV-1 infectious cycle. Although the CD4-binding site has been extensively characterized …

Electron microscopy of biological matter uses three different imaging modalities:(a) electron
crystallography,(b) single-particle analysis, and (c) electron tomography. Ideally, these …

Historically, the main barrier to membrane protein investigations has been the tendency of
membrane proteins to aggregate (due to their hydrophobic nature), in aqueous solution as …