[HTML][HTML] Therapeutic glycoprotein production in mammalian cells

ME Lalonde, Y Durocher - Journal of biotechnology, 2017 - Elsevier
Over the last years, the biopharmaceutical industry has significantly turned its biologics
production towards mammalian cell expression systems. The presence of glycosylation …

The art of CHO cell engineering: A comprehensive retrospect and future perspectives

S Fischer, R Handrick, K Otte - Biotechnology advances, 2015 - Elsevier
Chinese hamster ovary (CHO) cells represent the most frequently applied host cell system
for industrial manufacturing of recombinant protein therapeutics. CHO cells are capable of …

RNA interference functions as an antiviral immunity mechanism in mammals

Y Li, J Lu, Y Han, X Fan, SW Ding - Science, 2013 - science.org
Diverse eukaryotic hosts produce virus-derived small interfering RNAs (siRNAs) to direct
antiviral immunity by RNA interference (RNAi). However, it remains unknown whether the …

The emerging CHO systems biology era: harnessing the 'omics revolution for biotechnology

HF Kildegaard, D Baycin-Hizal, NE Lewis… - Current opinion in …, 2013 - Elsevier
Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals
because of their capacity to correctly fold and post-translationally modify recombinant …

An'omics approach towards CHO cell engineering

P Datta, RJ Linhardt… - Biotechnology and …, 2013 - Wiley Online Library
Chinese hamster ovarian cells (CHO) cells have been extensively utilized for industrial
production of biopharmaceutical products, such as monoclonal antibodies, human growth …

Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures

Z Du, D Treiber, JD McCarter… - Biotechnology and …, 2015 - Wiley Online Library
The continued need to improve therapeutic recombinant protein productivity has led to
ongoing assessment of appropriate strategies in the biopharmaceutical industry to establish …

Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7

N Barron, N Kumar, N Sanchez, P Doolan… - Journal of …, 2011 - Elsevier
The efficient production of recombinant proteins by Chinese Hamster Ovary (CHO) cells in
modern bioprocesses is often augmented by the use of proliferation control strategies. The …

Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing

J Becker, M Hackl, O Rupp, T Jakobi, J Schneider… - Journal of …, 2011 - Elsevier
The pyrosequencing technology from 454 Life Sciences and a novel assembly approach for
cDNA sequences with the Newbler Assembler were used to achieve a major step forward to …

[HTML][HTML] Next-generation sequencing of the Chinese hamster ovary microRNA transcriptome: Identification, annotation and profiling of microRNAs as targets for …

M Hackl, T Jakobi, J Blom, D Doppmeier… - Journal of …, 2011 - Elsevier
Chinese hamster ovary (CHO) cells are the predominant cell factory for the production of
recombinant therapeutic proteins. Nevertheless, the lack in publicly available sequence …

[HTML][HTML] CHO microRNA engineering is growing up: Recent successes and future challenges

V Jadhav, M Hackl, A Druz, S Shridhar, CY Chung… - Biotechnology …, 2013 - Elsevier
Abstract microRNAs with their ability to regulate complex pathways that control cellular
behavior and phenotype have been proposed as potential targets for cell engineering in the …