Advances in ultrahigh-throughput screening for directed enzyme evolution

U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

Metagenomics: application of genomics to uncultured microorganisms

J Handelsman - Microbiology and molecular biology reviews, 2004 - Am Soc Microbiol
Metagenomics (also referred to as environmental and community genomics) is the genomic
analysis of microorganisms by direct extraction and cloning of DNA from an assemblage of …

Industrial applications of microbial lipases

F Hasan, AA Shah, A Hameed - Enzyme and Microbial technology, 2006 - Elsevier
Lipases are a class of enzymes which catalyse the hydrolysis of long chain triglycerides.
Microbial lipases are currently receiving much attention with the rapid development of …

Metagenomics: genomic analysis of microbial communities

CS Riesenfeld, PD Schloss, J Handelsman - Annu. Rev. Genet., 2004 - annualreviews.org
▪ Abstract Uncultured microorganisms comprise the majority of the planet's biological
diversity. Microorganisms represent two of the three domains of life and contain vast …

The metagenomics of soil

R Daniel - Nature reviews microbiology, 2005 - nature.com
Phylogenetic surveys of soil ecosystems have shown that the number of prokaryotic species
found in a single sample exceeds that of known cultured prokaryotes. Soil metagenomics …

Characterization of a biogas-producing microbial community by short-read next generation DNA sequencing

R Wirth, E Kovács, G Maróti, Z Bagi, G Rákhely… - Biotechnology for …, 2012 - Springer
Background Renewable energy production is currently a major issue worldwide. Biogas is a
promising renewable energy carrier as the technology of its production combines the …

Cleaning up with genomics: applying molecular biology to bioremediation

DR Lovley - Nature Reviews Microbiology, 2003 - nature.com
Bioremediation has the potential to restore contaminated environments inexpensively yet
effectively, but a lack of information about the factors controlling the growth and metabolism …

Biotechnological prospects from metagenomics

PD Schloss, J Handelsman - Current opinion in biotechnology, 2003 - Elsevier
The recognition that most microorganisms in the environment cannot be cultured by
standard methods stimulated the development of metagenomics, which is the genomic …

Metagenomics–the key to the uncultured microbes

WR Streit, RA Schmitz - Current opinion in microbiology, 2004 - Elsevier
It is widely accepted that up to 99.8% of the microbes present in many environments are not
readily culturable.'Metagenome technology'tries to overcome this bottleneck by developing …

Metagenomics and industrial applications

P Lorenz, J Eck - Nature Reviews Microbiology, 2005 - nature.com
Different industries have different motivations to probe the enormous resource that is
uncultivated microbial diversity. Currently, there is a global political drive to promote white …