Crop improvement by genome editing involves the targeted alteration of genes to improve
plant traits, such as stress tolerance, disease resistance or nutritional content. Techniques …

The CRISPR-Cas systems have shown tremendous promise as heterologous tools for
genome editing in various prokaryotes. However, the perturbation of DNA homeostasis and …

CRISPR-based genome editing technology is revolutionizing prokaryotic research, but it has
been rarely studied in bacterial plant pathogens. Here, we have developed a targeted …

Thermus thermophilus is an attractive species in the bioindustry due to its valuable natural
products, abundant thermophilic enzymes, and promising fermentation capacities. However …

Abstract This research combined Whole-Genome sequencing, intraspecific comparative
genomics and transposon mutagenesis to investigate the menaquinone-7 (MK-7) synthesis …

Lactobacilli such as Lacticaseibacillus (Lcb) paracasei are generally regarded as safe and
health-promoting microbes, and have been widely applied in food and pharmaceutical …

The CRISPR-Cas system functions as an adaptive immune mechanism in archaea and
bacteria, providing defense against the invasion of foreign nucleic acids. Most CRISPR-Cas …

There are six major types of CRISPR-Cas systems that provide adaptive immunity in
bacteria and archaea against invasive genetic elements. The discovery of CRISPR-Cas …

Parageobacillus thermoglucosidasius is emerging as a highly promising thermophilic
organism for metabolic engineering. The utilization of CRISPR-Cas technologies has …

Enterococci are members of the microbiota of humans and other animals. They can also be
found in the environment, associated with food, healthcare infections, and hospital settings …