Thermal stability enhancement: Fundamental concepts of protein engineering strategies to manipulate the flexible structure
Increasing the temperature by just a few degrees may lead to structural perturbation or
unfolding of the protein and consequent loss of function. The concepts of flexibility and …
unfolding of the protein and consequent loss of function. The concepts of flexibility and …
[HTML][HTML] Protein disulfide engineering
Improving the stability of proteins is an important goal in many biomedical and industrial
applications. A logical approach is to emulate stabilizing molecular interactions found in …
applications. A logical approach is to emulate stabilizing molecular interactions found in …
Enhanced enzyme kinetic stability by increasing rigidity within the active site
Y Xie, J An, G Yang, G Wu, Y Zhang, L Cui… - Journal of Biological …, 2014 - ASBMB
Enzyme stability is an important issue for protein engineers. Understanding how rigidity in
the active site affects protein kinetic stability will provide new insight into enzyme …
the active site affects protein kinetic stability will provide new insight into enzyme …
Engineering proteins for thermostability through rigidifying flexible sites
Engineering proteins for thermostability is an exciting and challenging field since it is critical
for broadening the industrial use of recombinant proteins. Thermostability of proteins arises …
for broadening the industrial use of recombinant proteins. Thermostability of proteins arises …
In vivo monitoring of peripheral circadian clocks in the mouse
Y Tahara, H Kuroda, K Saito, Y Nakajima, Y Kubo… - Current Biology, 2012 - cell.com
The mammalian circadian system is comprised of a central clock in the suprachiasmatic
nucleus (SCN) and a network of peripheral oscillators located in all of the major organ …
nucleus (SCN) and a network of peripheral oscillators located in all of the major organ …
Split-luciferase complementary assay: applications, recent developments, and future perspectives
Bioluminescent systems are considered as potent reporter systems for bioanalysis since
they have specific characteristics, such as relatively high quantum yields and photon …
they have specific characteristics, such as relatively high quantum yields and photon …
Molecular enigma of multicolor bioluminescence of firefly luciferase
S Hosseinkhani - Cellular and Molecular Life Sciences, 2011 - Springer
Firefly luciferase-catalyzed reaction proceeds via the initial formation of an enzyme-bound
luciferyl adenylate intermediate. The chemical origin of the color modulation in firefly …
luciferyl adenylate intermediate. The chemical origin of the color modulation in firefly …
Efficient photocatalytic H2O2 production by using unstable SO functional groups as oxygen adsorption and active sites in shuttlecock waste-derived N, S-doped …
H Qin, K Sun, P Hao, H Yuan, Y Shen, A Bian, Y Cui… - Journal of …, 2024 - Elsevier
The growing demand for environmentally friendly hydrogen peroxide (H 2 O 2) production
has fueled extensive research into synthesis strategy for photocatalysis though the use of …
has fueled extensive research into synthesis strategy for photocatalysis though the use of …
The role of proline substitutions within flexible regions on thermostability of luciferase
Improving the stability of firefly luciferase has been a critical issue for its wider industrial
applications. Studies about hyperthermophile proteins show that flexibility could be an …
applications. Studies about hyperthermophile proteins show that flexibility could be an …
SpyTag/SpyCatcher cyclization enhances the thermostability of firefly luciferase
M Si, Q Xu, L Jiang, H Huang - PLoS One, 2016 - journals.plos.org
SpyTag can spontaneously form a covalent isopeptide bond with its protein partner
SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing …
SpyCatcher. Firefly luciferase from Photinus pyralis was cyclized in vivo by fusing …