Rational engineering of biological systems is often complicated by the complex but
unwanted interactions between cellular components at multiple levels. Here we address this …

Summary CRISPR RNAs (crRNAs) and Cas proteins work together to provide prokaryotes
with adaptive immunity against genetic invaders like bacteriophages and plasmids …

Engineered living materials combine the advantages of biological and synthetic systems by
leveraging genetic and metabolic programming to control material-wide properties. Here, we …

Mitigating unintended interferences between circuits and host cells is key to realize
applications of synthetic regulatory systems both for bacteria and mammalian cells. Here, we …

Unlike their natural counterparts, synthetic genetic circuits are usually fragile in the face of
environmental perturbations and genetic mutations. Several theoretical robust genetic …

Synthetic Biology aims to create predictable biological circuits and fully operational
biological systems. Although there are methods to create more stable oscillators, such as …

Overexpression of a single enzyme in a multigene heterologous pathway may be out of
balance with the other enzymes in the pathway, leading to accumulated toxic intermediates …

Although the principles of synthetic biology were initially established in model bacteria,
microbial producers, extremophiles and gut microbes have now emerged as valuable …

Escherichia coli is a common chassis for synthetic gene circuit studies. In addition to the
dose–response of synthetic gene circuits, the analysis of dynamic responses is also an …

One of the purposes of synthetic biology is to develop rational methods that accelerate the
design of genetic circuits, saving time and effort spent on experiments and providing reliably …