The lysis-lysogeny decision of bacteriophage lambda (λ) is a paradigm for developmental
genetic networks. There are three key features, which characterize the network. First, after …

After an illustrious history as one of the primary tools that established the foundations of
molecular biology, bacteriophage research is now undergoing a renaissance in which the …

The mammalian gut is a dynamic community of symbiotic microbes that interact with the host
to impact health, disease, and metabolism. We constructed engineered bacteria that survive …

We have constructed a set of plasmids that can be used to express recombineering
functions in some gram-negative bacteria, thereby facilitating in vivo genetic manipulations …

Transcriptional interference with convergent transcription from face-to-face promoters is a
potentially important form of gene regulation in all organisms. Using LacZ reporter studies …

The temperature inducible expression system, based on the pL and/or pR phage lambda
promoters regulated by the thermolabile cI857 repressor has been widely use to produce …

In vivo mapping of transcription-factor binding to the transcriptional output of the regulated
gene is hindered by probabilistic promoter occupancy, the presence of multiple gene copies …

When we think about viruses we tend to consider ones that afflict humans—such as those
that cause influenza, HIV, and Ebola. Yet, vastly more viruses infect single-celled microbes …

Overexpression of a drug's molecular target often increases drug resistance, offering a
pathway for adaptive evolution and a tool for target identification. It is unclear though why …

Effective repression of cI transcription from P RM by the bacteriophage λ CI repressor
requires binding sites (OL) located 2.4 kb from the promoter. A CI tetramer bound to O L1. O …