Wettability-patterned microchip for emerging biomedical materials and technologies

Y Li, BF Liu, X Zhang - Materials Today, 2021 - Elsevier
Microchip has long been studied and facilitated recent investigations in multiple biomedical
and material fields. The advances in functional materials triggered several leaps in the …

Understanding Molecular Transfer between Aqueous Phase and Reverse Micelles and Its Application to Bioassays in Microfluidics

M Fukuyama - Bulletin of the Chemical Society of Japan, 2023 - academic.oup.com
Micrometer-sized water-in-oil droplets (microdroplets) are attracting attention as carriers for
the high-throughput parallel analysis of trace samples. By confining trace amounts of water …

Nanoparticle Assembly at the Water–Oil Interface Induced by Spontaneous Emulsification for Microdroplet Immunoassay

M Fukuyama, K Kubota, A Hibara - Langmuir, 2023 - ACS Publications
Micron-sized water-in-oil droplets (microdroplets) have been used for various biochemical
analyses. Many studies have been reported on immunoassays using microdroplets because …

Resistive Pulse Sensing on a Capillary-Assisted Microfluidic Platform for On-Site Single-Particle Analyses

T Shimada, K Fujino, T Yasui, N Kaji, Y Ueda… - Analytical …, 2023 - ACS Publications
Capillary-assisted flow is valuable for utilizing microfluidics-based electrical sensing
platforms at on-site locations by simplifying microfluidic operations and system construction; …

Monodisperse liposomes with femtoliter volume enable quantitative digital bioassays of membrane transporters and cell-free gene expression

N Soga, A Ota, K Nakajima, R Watanabe, H Ueno… - ACS …, 2020 - ACS Publications
Digital bioassays have emerged as a new category of bioanalysis. However, digital
bioassays for membrane transporter proteins have not been well established yet despite …

High-throughput single-molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules

R Watanabe, T Komatsu, S Sakamoto, Y Urano, H Noji - Lab on a Chip, 2018 - pubs.rsc.org
Micro-reactor arrays enable highly sensitive and quantitative bioassays at a single-molecule
level. Accordingly, they are widely used for sensitive “digital” bioassays, eg, digital PCR and …

Sensitivity-improved blocking agent-free fluorescence polarization assay through surface modification using polyethylene glycol

H Liu, M Fukuyama, Y Ogura, M Kasuya, S Onose… - Analyst, 2024 - pubs.rsc.org
Fluorescence polarization (FP) assays are widely used to quantify biomolecules, and their
combination with microfluidic devices has the potential for application in onsite analysis …

Controlling water transport between micelles and aqueous microdroplets during sample enrichment

M Fukuyama, L Zhou, T Okada, KV Simonova… - Analytica Chimica …, 2021 - Elsevier
Droplet microfluidics technologies have advanced rapidly, but enrichment in droplets has
still been difficult. To deterministically control the droplet enrichment, the water transport from …

Light fueled mixing in open surface droplet microfluidics for rapid probe preparation

D Li, R Chen, X Zhu, D Ye, Y Yang, W Li, H Li… - Physical Chemistry …, 2021 - pubs.rsc.org
In this study, a contactless, flexible, and interference-free light fueled method has been
developed to enhance the mixing between the ssDNA and dynabeads in a droplet, which …

Transport of Oligopeptide from Aqueous Phase to Span 80 Reverse Micelles in Microdroplet Array

M Fukuyama, M Suto, A Hibara - Analytical Sciences, 2021 - Springer
The partitioning of water and tetramethylrhodamine-conjugated-10-residue oligopeptides
from the aqueous phase of microdroplets into Span 80 reverse micelles was observed by …