This review deals with phytocystatins, focussing on their potential role as defence proteins
against phytophagous arthropods. Information about the evolutionary, molecular and …

Sugarcane is one of the most important agricultural crops in the world. However, pathogen
infection and herbivore attack cause constant losses in yield. Plants respond to pathogen …

The antimicrobial and anticaries effects of CaneCPI-5 were evaluated. Ninety bovine
enamel samples were treated for 60 s with either phosphate-buffered-saline (PBS), 0.12 …

The effect of solutions and gels containing a sugarcane-derived cystatin (CaneCPI-5) on the
protection against enamel and dentin erosion in vitro was evaluated. Bovine enamel and …

Abstract Domain swapping is the process by which identical monomeric proteins exchange
structural elements to generate dimers/oligomers. Although engineered domain swapping is …

Genome sequencing has led to the identification of many proteins, which had not been
recognized before. In consequence, the basic set of human proteins is generally known. Far …

Phytocystatins are tight-binding cysteine protease inhibitors produced by plants. The first
phytocystatin described was isolated from Oryza sativa and, since then, cystatins from …

Two cysteine proteinase inhibitors from cowpea, VuCys1 and VuCys2, were produced in E.
coli ArcticExpress (DE3). The recombinant products strongly inhibited papain and …

Dozens of studies have assessed the practical value of plant cystatins as ectopic inhibitors
of Cys proteases in biological systems. The potential of these proteins in crop protection to …

Cystatins encode a high functional variability not only because of their ability to inhibit
different classes of proteases but also because of their propensity to form oligomers and …