The development of new ion-activation/dissociation methods continues to be one of the most
active areas of mass spectrometry owing to the broad applications of tandem mass …

Protein phosphorylation is a major regulator of protein function and biological outcomes.
This was first recognized through functional biochemical experiments, and in the past …

The emergence of proteomics has led to major technological advances in mass
spectrometry (MS). These advancements not only benefitted MS‐based high‐throughput …

Cells organize their actions partly through tightly controlled protein-protein interactions—
collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to …

Pioneering work by, among others, the groups of Hunt and Mann at the start of this century
opened up the era of mass spectrometry (MS)-based phosphoproteomics. 1− 3 Through …

The proteome, or collection of proteoforms expressed in a biological system, is dynamic and
heterogeneous. As our appreciation for the complexity of the proteome has evolved, so have …

Sphingolipids serve not only as components of cellular membranes but also as bioactive
mediators of numerous cellular functions. As the biological activities of these lipids are …

Infrared multiple photon dissociation (IRMPD) spectroscopy allows for the derivation of the
vibrational fingerprint of molecular ions under tandem mass spectrometry (MS/MS) …

Compared to traditional collision induced dissociation methods, electron capture
dissociation (ECD) provides more comprehensive characterization of large peptides and …

Post-translational modifications (PTMs) on intact histones play a major role in regulating
chromatin dynamics and influence biological processes such as DNA transcription …