Chemical exchange in biomacromolecules: past, present, and future

AG Palmer III - Journal of magnetic resonance, 2014 - Elsevier
The perspective reviews quantitative investigations of chemical exchange phenomena in
proteins and other biological macromolecules using NMR spectroscopy, particularly …

A guide to large-scale RNA sample preparation

L Baronti, H Karlsson, M Marušič, K Petzold - Analytical and bioanalytical …, 2018 - Springer
RNA is becoming more important as an increasing number of functions, both regulatory and
enzymatic, are being discovered on a daily basis. As the RNA boom has just begun, most …

2′-O-Methylation can increase the abundance and lifetime of alternative RNA conformational states

H Abou Assi, AK Rangadurai, H Shi, B Liu… - Nucleic acids …, 2020 - academic.oup.com
O-Methyl (Nm) is a highly abundant post-transcriptional RNA modification that plays
important biological roles through mechanisms that are not entirely understood. There is …

[HTML][HTML] m1A and m1G disrupt A-RNA structure through the intrinsic instability of Hoogsteen base pairs

H Zhou, IJ Kimsey, EN Nikolova… - Nature structural & …, 2016 - nature.com
The B-DNA double helix can dynamically accommodate GC and AT base pairs in either
Watson–Crick or Hoogsteen configurations. Here, we show that G-C+ (in which+ indicates …

Characterizing micro-to-millisecond chemical exchange in nucleic acids using off-resonance R1ρ relaxation dispersion

A Rangadurai, ES Szymaski, IJ Kimsey, H Shi… - Progress in nuclear …, 2019 - Elsevier
This review describes off-resonance R 1ρ relaxation dispersion NMR methods for
characterizing microsecond-to-millisecond chemical exchange in uniformly 13 C/15 N …

RNA dynamics by NMR spectroscopy

M Marušič, J Schlagnitweit, K Petzold - ChemBioChem, 2019 - Wiley Online Library
An ever‐increasing number of functional RNAs require a mechanistic understanding. RNA
function relies on changes in its structure, so‐called dynamics. To reveal dynamic processes …

RNA binding and chaperone activity of the E. coli cold-shock protein CspA

E Rennella, T Sára, M Juen, C Wunderlich… - Nucleic Acids …, 2017 - academic.oup.com
Ensuring the correct folding of RNA molecules in the cell is of major importance for a large
variety of biological functions. Therefore, chaperone proteins that assist RNA in adopting …

Characterizing Slow Chemical Exchange in Nucleic Acids by Carbon CEST and Low Spin-Lock Field R NMR Spectroscopy

B Zhao, AL Hansen, Q Zhang - Journal of the American Chemical …, 2014 - ACS Publications
Quantitative characterization of dynamic exchange between various conformational states
provides essential insights into the molecular basis of many regulatory RNA functions. Here …

A Stably Protonated Adenine Nucleotide with a Highly Shifted pKa Value Stabilizes the Tertiary Structure of a GTP‐Binding RNA Aptamer

AC Wolter, AK Weickhmann, AH Nasiri… - Angewandte Chemie …, 2017 - Wiley Online Library
RNA tertiary structure motifs are stabilized by a wide variety of hydrogen‐bonding
interactions. Protonated A and C nucleotides are normally not considered to be suitable …

2′-O-Trifluoromethylated RNA–a powerful modification for RNA chemistry and NMR spectroscopy

M Himmelstoß, K Erharter, E Renard, E Ennifar… - Chemical …, 2020 - pubs.rsc.org
New RNA modifications are needed to advance our toolbox for targeted manipulation of
RNA. In particular, the development of high-performance reporter groups facilitating …