Synthetic biology tools for novel secondary metabolite discovery in Streptomyces
Streptomyces are attractive microbial cell factories that have industrial capability to produce
a wide array of bioactive secondary metabolites. However, the genetic potential of the …
a wide array of bioactive secondary metabolites. However, the genetic potential of the …
Systems and synthetic biology to elucidate secondary metabolite biosynthetic gene clusters encoded in Streptomyces genomes
Covering: 2010 to 2020 Over the last few decades, Streptomyces have been extensively
investigated for their ability to produce diverse bioactive secondary metabolites. Recent …
investigated for their ability to produce diverse bioactive secondary metabolites. Recent …
Biosynthesis of polycyclopropanated high energy biofuels
Cyclopropane-functionalized hydrocarbons are excellent fuels due their high energy
density. However, the organic synthesis of these molecules is challenging. In this work, we …
density. However, the organic synthesis of these molecules is challenging. In this work, we …
Molecular Toolkit for Gene Expression Control and Genome Modification in Rhodococcus opacus PD630
DM DeLorenzo, AG Rottinghaus, WR Henson… - ACS synthetic …, 2018 - ACS Publications
Rhodococcus opacus PD630 is a non-model Gram-positive bacterium that possesses
desirable traits for lignocellulosic biomass conversion. In particular, it has a relatively rapid …
desirable traits for lignocellulosic biomass conversion. In particular, it has a relatively rapid …
Synthetic biology advances and applications in the biotechnology industry: a perspective
L Katz, YY Chen, R Gonzalez… - Journal of Industrial …, 2018 - academic.oup.com
Synthetic biology is a logical extension of what has been called recombinant DNA (rDNA)
technology or genetic engineering since the 1970s. As rDNA technology has been the driver …
technology or genetic engineering since the 1970s. As rDNA technology has been the driver …
Activating natural product synthesis using CRISPR interference and activation systems in Streptomyces
A Ameruoso, MC Villegas Kcam, KP Cohen… - Nucleic Acids …, 2022 - academic.oup.com
The rise of antibiotic-resistant bacteria represents a major threat to global health, creating an
urgent need to discover new antibiotics. Natural products derived from the genus …
urgent need to discover new antibiotics. Natural products derived from the genus …
A Streptomyces venezuelae Cell-Free Toolkit for Synthetic Biology
Prokaryotic cell-free coupled transcription–translation (TX-TL) systems are emerging as a
powerful tool to examine natural product biosynthetic pathways in a test tube. The key …
powerful tool to examine natural product biosynthetic pathways in a test tube. The key …
Streptomyces venezuelae TX‐TL – a next generation cell‐free synthetic biology tool
Streptomyces venezuelae is a promising chassis in synthetic biology for fine chemical and
secondary metabolite pathway engineering. The potential of S. venezuelae could be further …
secondary metabolite pathway engineering. The potential of S. venezuelae could be further …
Short-chain ketone production by engineered polyketide synthases in Streptomyces albus
S Yuzawa, M Mirsiaghi, R Jocic, T Fujii… - Nature …, 2018 - nature.com
Microbial production of fuels and commodity chemicals has been performed primarily using
natural or slightly modified enzymes, which inherently limits the types of molecules that can …
natural or slightly modified enzymes, which inherently limits the types of molecules that can …
Recent advances in strategies for the cloning of natural product biosynthetic gene clusters
W Wang, G Zheng, Y Lu - Frontiers in bioengineering and …, 2021 - frontiersin.org
Microbial natural products (NPs) are a major source of pharmacological agents. Most NPs
are synthesized from specific biosynthetic gene clusters (BGCs). With the rapid increase of …
are synthesized from specific biosynthetic gene clusters (BGCs). With the rapid increase of …