Machine learning-guided protein engineering

P Kouba, P Kohout, F Haddadi, A Bushuiev… - ACS …, 2023 - ACS Publications
Recent progress in engineering highly promising biocatalysts has increasingly involved
machine learning methods. These methods leverage existing experimental and simulation …

Machine learning-enabled retrobiosynthesis of molecules

T Yu, AG Boob, MJ Volk, X Liu, H Cui, H Zhao - Nature Catalysis, 2023 - nature.com
Retrobiosynthesis provides an effective and sustainable approach to producing functional
molecules. The past few decades have witnessed a rapid expansion of biosynthetic …

Efficient evolution of human antibodies from general protein language models

BL Hie, VR Shanker, D Xu, TUJ Bruun… - Nature …, 2024 - nature.com
Natural evolution must explore a vast landscape of possible sequences for desirable yet
rare mutations, suggesting that learning from natural evolutionary strategies could guide …

Learning protein fitness models from evolutionary and assay-labeled data

C Hsu, H Nisonoff, C Fannjiang, J Listgarten - Nature biotechnology, 2022 - nature.com
Abstract Machine learning-based models of protein fitness typically learn from either
unlabeled, evolutionarily related sequences or variant sequences with experimentally …

ECNet is an evolutionary context-integrated deep learning framework for protein engineering

Y Luo, G Jiang, T Yu, Y Liu, L Vo, H Ding, Y Su… - Nature …, 2021 - nature.com
Abstract Machine learning has been increasingly used for protein engineering. However,
because the general sequence contexts they capture are not specific to the protein being …

Self-play reinforcement learning guides protein engineering

Y Wang, H Tang, L Huang, L Pan, L Yang… - Nature Machine …, 2023 - nature.com
Designing protein sequences towards desired properties is a fundamental goal of protein
engineering, with applications in drug discovery and enzymatic engineering. Machine …

Combining chemistry and protein engineering for new-to-nature biocatalysis

DC Miller, SV Athavale, FH Arnold - Nature Synthesis, 2022 - nature.com
Biocatalysis, the application of enzymes to solve synthetic problems of human import, has
blossomed into a powerful technology for chemical innovation. In the past decade, a …

Unsupervised evolution of protein and antibody complexes with a structure-informed language model

VR Shanker, TUJ Bruun, BL Hie, PS Kim - Science, 2024 - science.org
Large language models trained on sequence information alone can learn high-level
principles of protein design. However, beyond sequence, the three-dimensional structures of …

Machine learning-guided co-optimization of fitness and diversity facilitates combinatorial library design in enzyme engineering

K Ding, M Chin, Y Zhao, W Huang, BK Mai… - Nature …, 2024 - nature.com
The effective design of combinatorial libraries to balance fitness and diversity facilitates the
engineering of useful enzyme functions, particularly those that are poorly characterized or …

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

M Gantz, S Neun, EJ Medcalf, LD van Vliet… - Chemical …, 2023 - ACS Publications
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …