Faster, sharper, and deeper: structured illumination microscopy for biological imaging

Y Wu, H Shroff - Nature methods, 2018 - nature.com
Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live
specimens. We review recent technical advances in SR-SIM, with emphasis on imaging …

What spatial light modulators can do for optical microscopy

C Maurer, A Jesacher, S Bernet… - Laser & Photonics …, 2011 - Wiley Online Library
With the availability of high‐resolution miniature spatial light modulators (SLMs) new
methods in optical microscopy have become feasible. The SLMs discussed in this review …

Super-resolution video microscopy of live cells by structured illumination

P Kner, BB Chhun, ER Griffis, L Winoto… - Nature …, 2009 - nature.com
Structured-illumination microscopy can double the resolution of the widefield fluorescence
microscope but has previously been too slow for dynamic live imaging. Here we …

DMD-based LED-illumination super-resolution and optical sectioning microscopy

D Dan, M Lei, B Yao, W Wang, M Winterhalder… - Scientific reports, 2013 - nature.com
Abstract Super-resolution three-dimensional (3D) optical microscopy has incomparable
advantages over other high-resolution microscopic technologies, such as electron …

Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination

R Fiolka, L Shao, EH Rego… - Proceedings of the …, 2012 - National Acad Sciences
Previous implementations of structured-illumination microscopy (SIM) were slow or
designed for one-color excitation, sacrificing two unique and extremely beneficial aspects of …

Structured illumination microscopy

M Saxena, G Eluru, SS Gorthi - Advances in Optics and Photonics, 2015 - opg.optica.org
Illumination plays an important role in optical microscopy. Köhler illumination, introduced
more than a century ago, has been the backbone of optical microscopes. The last few …

FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli

Z Lyu, A Yahashiri, X Yang, JW McCausland… - Nature …, 2022 - nature.com
FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG)
by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here …

Patchwork organization of the yeast plasma membrane into numerous coexisting domains

F Spira, NS Mueller, G Beck, P Von Olshausen… - Nature cell …, 2012 - nature.com
The plasma membrane is made up of lipids and proteins, and serves as an active interface
between the cell and its environment. Many plasma-membrane proteins are laterally …

A new wave of cellular imaging

D Toomre, J Bewersdorf - Annual review of cell and …, 2010 - annualreviews.org
Fluorescence imaging methods that push or break the diffraction limit of resolution
(approximately 200 nm) have grown explosively. These super-resolution nanoscopy …

Phase optimisation for structured illumination microscopy

K Wicker, O Mandula, G Best, R Fiolka… - Optics express, 2013 - opg.optica.org
Structured illumination microscopy can achieve super-resolution in fluorescence imaging.
The sample is illuminated with periodic light patterns, and a series of images are acquired …