Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches

ZA Parray, F Ahmad, MF Alajmi, A Hussain… - Scientific reports, 2021 - nature.com
One of the significant proteins that have attracted research groups due to virtue of being a
potent selective anticancer drug target and property of triggering apoptosis upon release in …

Macromolecular crowding: how shape and interaction affect the structure, function, conformational dynamics and relative domain movement of a multi-domain protein

N Das, P Sen - Physical Chemistry Chemical Physics, 2022 - pubs.rsc.org
The cellular environment is crowded by macromolecules of various sizes, shapes, and
charges, which modulate protein structure, function and dynamics. Herein, we contemplated …

Macromolecular crowding modulates α-synuclein amyloid fiber growth

I Horvath, R Kumar, P Wittung-Stafshede - Biophysical Journal, 2021 - cell.com
The crowdedness of living cells, hundreds of milligrams per milliliter of macromolecules,
may affect protein folding, function, and misfolding. Still, such processes are most often …

Biophysical elucidation of fibrillation inhibition by sugar osmolytes in α-lactalbumin: multispectroscopic and molecular docking approaches

S Bashir, A Shamsi, F Ahmad, MI Hassan, MA Kamal… - Acs Omega, 2020 - ACS Publications
Protein aggregation is among the most challenging new frontiers in protein chemistry as well
as in molecular medicine and has direct implications in protein misfolding. This study …

[HTML][HTML] Protein folding mechanism revealed by single-molecule force spectroscopy experiments

H Sun, Z Guo, H Hong, P Yu, Z Xue, H Chen - Biophysics Reports, 2021 - ncbi.nlm.nih.gov
Force spectroscopy experiments use mechanical force as a control factor to regulate the
folding and unfolding process of proteins. Atomic force microscopy has been widely used to …

Quantitative evaluation of macromolecular crowding environment based on translational and rotational diffusion using polarization dependent fluorescence correlation …

J Yamamoto, A Matsui, F Gan, M Oura, R Ando… - Scientific reports, 2021 - nature.com
Macromolecular crowding (MMC) in cells is a hot topic in biology; therefore, well-
characterized measurement standards for the evaluation of the nano-environment in MMC …

Size-Dependent Interplay of Volume Exclusion Versus Soft Interactions: Cytochrome c in Macromolecular Crowded Environment

ZA Parray, F Ahmad, AA Chaudhary… - Frontiers in molecular …, 2022 - frontiersin.org
Even though there are a great number of possible conformational states, how a protein
generated as a linear unfolded polypeptide efficiently folds into its physiologically active …

Potential of Osmolytes as Diagnostic Biomarkers in Various Diseases

ZA Parray, IA Ahanger, A Islam - Cellular Osmolytes: From Chaperoning …, 2024 - Springer
Osmolytes are low-molecular-weight organic compounds that protect cells from extreme
stress conditions by regulating osmotic pressure and maintaining cellular homeostasis …

[HTML][HTML] Unveiling the Catalytic Roles of DsBBS1 and DsBBS2 in the Bibenzyl Biosynthesis of Dendrobium sinense

L Liu, H You, L Ye, Q Ou, Y Zhao, J Wang, J Niu - Molecules, 2024 - mdpi.com
Dendrobium sinense, an endemic medicinal herb in Hainan Island, is rich in bibenzyl
compounds. However, few studies have explored the molecular mechanisms of bibenzyl …

[HTML][HTML] Structural refolding and thermal stability of myoglobin in the presence of mixture of crowders: importance of various interactions for protein stabilization in …

ZA Parray, F Ahmad, MI Hassan, A Ahmed… - Molecules, 2021 - mdpi.com
The intracellular environment is overcrowded with a range of molecules (small and large),
all of which influence protein conformation. As a result, understanding how proteins fold and …