Metalloproteins catalyse some of the most complex and important processes in nature, such
as photosynthesis and water oxidation. An ultimate test of our knowledge of how …

The availability of vast gene repertoires from both natural sources (genomic and cDNA
libraries) and artificial sources (gene libraries) demands the development and application of …

Transition metal catalysts and enzymes possess unique and often complementary
properties that have made them important tools for chemical synthesis. The potential …

Ubiquitin (UB) transfer cascades consisting of E1, E2, and E3 enzymes constitute a complex
network that regulates a myriad of biologic processes by modifying protein substrates …

We report a method for in vitro selection of catalytically active enzymes from large libraries of
variants displayed on the surface of the yeast S. cerevisiae. Two libraries, each containing∼ …

E3 ubiquitin (UB) ligases E4B and carboxyl terminus of Hsc70-interacting protein (CHIP) use
a common U-box motif to transfer UB from E1 and E2 enzymes to their substrate proteins …

We report a high-throughput phage selection method to identify mutants of Sfp
phosphopantetheinyl transferase with altered substrate specificities from a large library of …

Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach
for the interrogation of the cellular and molecular logic of the nervous system. Herein, a …

The design of artificial hemoproteins that could catalyze selective oxidations using clean
oxidants such as O2 or H2O2 under ecocompatible conditions constitutes a real challenge …

We have developed a phage-display method for high-throughput mining of bacterial gene
clusters encoding the natural-product biosynthetic enzymes, polyketide synthases (PKSs) …