Speed, resolution and sensitivity of today's fluorescence bioimaging can be drastically
improved by fluorescent nanoparticles (NPs) that are many‐fold brighter than organic dyes …

Ever since their introduction two decades ago, single-molecule (SM) fluorescence methods
have matured and branched out to address numerous biological questions, which were …

We present a whole-cell fully dynamical kinetic model (WCM) of JCVI-syn3A, a minimal cell
with a reduced genome of 493 genes that has retained few regulatory proteins or small …

Cellular processes are carried out by many genes, and their study and optimization requires
multiple levers by which they can be independently controlled. The most common method is …

Because RNA lacks strong intrinsic fluorescence, it has proven challenging to track RNA
molecules in real time. To address this problem and to allow the purification of fluorescently …

The rules of nucleic acid base-pairing have been used to construct nanoscale architectures
and organize biomolecules, but little has been done to apply this technology in vivo. We …

Protein levels have been shown to vary substantially between individual cells in clonal
populations. In prokaryotes, the contribution to such fluctuations from the inherent …

Quantitative spatial distributions of ribosomes (S2‐YFP) and RNA polymerase (RNAP; β′‐
yGFP) in live Escherichia coli are measured by superresolution fluorescence microscopy. In …

We track the motion of individual fluorescently labeled mRNA molecules inside live E. coli
cells. We find that the motion is subdiffusive, with an exponent that is robust to physiological …

Highly fluorescent conjugated polymer dots were developed for demanding applications
such as fluorescence imaging in live cells. These nanoparticles exhibit small particle …