Applying a high voltage to a liquid passing through a capillary disintegrates the liquid
meniscus to generate a fine spray of charged droplets. As the droplets evaporate, polar …

Recent advances in efficiency and ease of implementation have rekindled interest in ion
mobility spectrometry, a technique that separates gas phase ions by their size and shape …

The dia-PASEF technology uses ion mobility separation to reduce signal interferences and
increase sensitivity in proteomic experiments. Here we present a two-dimensional peak …

The COVID-19 pandemic is an unprecedented global challenge, and point-of-care
diagnostic classifiers are urgently required. Here, we present a platform for ultra-high …

This protocol describes the use of TurboID and split-TurboID in proximity labeling
applications for mapping protein–protein interactions and subcellular proteomes in live …

Lactylation was initially discovered on human histones. Given its nascence, its occurrence
on nonhistone proteins and downstream functional consequences remain elusive. Here we …

Plants are essential for life and are extremely diverse organisms with unique molecular
capabilities. Here we present a quantitative atlas of the transcriptomes, proteomes and …

Defining the cellular response to pharmacological agents is critical for understanding the
mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate …

Single-cell proteomics by mass spectrometry (SCoPE-MS) is a recently introduced method
to quantify multiplexed single-cell proteomes. While this technique has generated great …

Although most cancer drugs modulate the activities of cellular pathways by changing
posttranslational modifications (PTMs), little is known regarding the extent and the time-and …