Protein phosphorylation: a major switch mechanism for metabolic regulation
Metabolism research is undergoing a renaissance because many diseases are increasingly
recognized as being characterized by perturbations in intracellular metabolic regulation …
recognized as being characterized by perturbations in intracellular metabolic regulation …
Phosphoproteomics in the age of rapid and deep proteome profiling
Protein phosphorylation is a post-translational modification (PTM) that orchestrates a diverse
array of cellular processes. Because this modification serves as a rapid and reversible …
array of cellular processes. Because this modification serves as a rapid and reversible …
Global detection of human variants and isoforms by deep proteome sequencing
An average shotgun proteomics experiment detects approximately 10,000 human proteins
from a single sample. However, individual proteins are typically identified by peptide …
from a single sample. However, individual proteins are typically identified by peptide …
Six alternative proteases for mass spectrometry–based proteomics beyond trypsin
Protein digestion using a dedicated protease represents a key element in a typical mass
spectrometry (MS)-based shotgun proteomics experiment. Up to now, digestion has been …
spectrometry (MS)-based shotgun proteomics experiment. Up to now, digestion has been …
An optimized shotgun strategy for the rapid generation of comprehensive human proteomes
This study investigates the challenge of comprehensively cataloging the complete human
proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics …
proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics …
Strong anion exchange‐mediated phosphoproteomics reveals extensive human non‐canonical phosphorylation
G Hardman, S Perkins, PJ Brownridge, CJ Clarke… - The EMBO …, 2019 - embopress.org
Phosphorylation is a key regulator of protein function under (patho) physiological conditions,
and defining site‐specific phosphorylation is essential to understand basic and disease …
and defining site‐specific phosphorylation is essential to understand basic and disease …
Building ProteomeTools based on a complete synthetic human proteome
We describe ProteomeTools, a project building molecular and digital tools from the human
proteome to facilitate biomedical research. Here we report the generation and multimodal …
proteome to facilitate biomedical research. Here we report the generation and multimodal …
The role of electron transfer dissociation in modern proteomics
(MS) and MS-driven proteomics have continued to develop as the premier tools for
characterizing proteome composition, structure, and function. 2 The new era of proteomics …
characterizing proteome composition, structure, and function. 2 The new era of proteomics …
An integrated workflow for crosslinking mass spectrometry
We present a concise workflow to enhance the mass spectrometric detection of crosslinked
peptides by introducing sequential digestion and the crosslink identification software xi …
peptides by introducing sequential digestion and the crosslink identification software xi …
An advanced strategy for comprehensive profiling of ADP-ribosylation sites using mass spectrometry-based proteomics
ADP-ribosylation is a widespread post-translational modification (PTM) with crucial functions
in many cellular processes. Here, we describe an in-depth ADP-ribosylome using our …
in many cellular processes. Here, we describe an in-depth ADP-ribosylome using our …