Systems and synthetic biology to elucidate secondary metabolite biosynthetic gene clusters encoded in Streptomyces genomes

N Lee, S Hwang, W Kim, Y Lee, JH Kim, S Cho… - Natural Product …, 2021 - pubs.rsc.org
Covering: 2010 to 2020 Over the last few decades, Streptomyces have been extensively
investigated for their ability to produce diverse bioactive secondary metabolites. Recent …

[HTML][HTML] Streptomyces as Microbial Chassis for Heterologous Protein Expression

S Hwang, Y Lee, JH Kim, G Kim, H Kim… - … in Bioengineering and …, 2021 - frontiersin.org
Heterologous production of recombinant proteins is gaining increasing interest in
biotechnology with respect to productivity, scalability, and wide applicability. The members …

[HTML][HTML] Prospective bacterial and fungal sources of hyaluronic acid: A review

EV Shikina, RA Kovalevsky, AI Shirkovskaya… - Computational and …, 2022 - Elsevier
The unique biological and rheological properties make hyaluronic acid a sought-after
material for medicine and cosmetology. Due to very high purity requirements for hyaluronic …

The design-build-test-learn cycle for metabolic engineering of streptomycetes

CM Whitford, P Cruz-Morales, JD Keasling… - Essays in …, 2021 - portlandpress.com
Streptomycetes are producers of a wide range of specialized metabolites of great medicinal
and industrial importance, such as antibiotics, antifungals, or pesticides. Having been the …

[HTML][HTML] RiboRid: A low cost, advanced, and ultra-efficient method to remove ribosomal RNA for bacterial transcriptomics

D Choe, R Szubin, S Poudel, A Sastry, Y Song… - PLoS …, 2021 - journals.plos.org
RNA sequencing techniques have enabled the systematic elucidation of gene expression
(RNA-Seq), transcription start sites (differential RNA-Seq), transcript 3′ ends (Term-Seq) …

A comparative metabologenomic approach reveals mechanistic insights into Streptomyces antibiotic crypticity

Y Qi, KK Nepal, JAV Blodgett - Proceedings of the National …, 2021 - National Acad Sciences
Streptomyces genomes harbor numerous, biosynthetic gene clusters (BGCs) encoding for
drug-like compounds. While some of these BGCs readily yield expected products, many do …

Development of highly characterized genetic bioparts for efficient gene expression in CO2-fixing Eubacterium limosum

Y Song, J Bae, S Jin, H Lee, S Kang, J Lee, J Shin… - Metabolic …, 2022 - Elsevier
Acetogenic bacteria demonstrate industrial potential for utilizing carbon dioxide (CO 2) for
biochemical production using the Wood-Ljungdahl pathway. However, the metabolic …

Different Regulatory Modes of Synechocystis sp. PCC 6803 in Response to Photosynthesis Inhibitory Conditions

SH Cho, Y Jeong, SJ Hong, H Lee, HK Choi, DM Kim… - Msystems, 2021 - Am Soc Microbiol
Cyanobacteria are promising industrial platforms owing to their ability to produce diverse
natural secondary metabolites and nonnative value-added biochemicals from CO2 and light …

[HTML][HTML] Transcriptome and translatome profiles of Streptomyces species in different growth phases

W Kim, S Hwang, N Lee, Y Lee, S Cho, B Palsson… - Scientific data, 2020 - nature.com
Streptomyces are efficient producers of various bioactive compounds, which are mostly
synthesized by their secondary metabolite biosynthetic gene clusters (smBGCs). The …

Synthetic 3′-UTR valves for optimal metabolic flux control in Escherichia coli

D Choe, K Kim, M Kang, SG Lee, S Cho… - Nucleic Acids …, 2022 - academic.oup.com
As the design of genetic circuitry for synthetic biology becomes more sophisticated, diverse
regulatory bioparts are required. Despite their importance, well-characterized 3 …