Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects

KC Beng, RT Corlett - Biodiversity and conservation, 2020 - Springer
Conserving biodiversity in the face of ever-increasing human pressure is hampered by our
lack of basic information on species occurrence, distribution, abundance, habitat …

Environmental DNA metabarcoding: A novel method for biodiversity monitoring of marine fish communities

M Miya - Annual Review of Marine Science, 2022 - annualreviews.org
Environmental DNA (eDNA) is genetic material that has been shed from macroorganisms. It
has received increased attention as an indirect marker for biodiversity monitoring. This …

MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

[图书][B] A practical guide to DNA-based methods for biodiversity assessment.

K Bruce, RC Blackman, SJ Bourlat, M Hellström… - 2021 - repository.oceanbestpractices.org
This book represents a synthesis of knowledge and best practice in the field of DNA-based
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …

Biases in bulk: DNA metabarcoding of marine communities and the methodology involved

LM van der Loos, R Nijland - Molecular Ecology, 2021 - Wiley Online Library
With the growing anthropogenic pressure on marine ecosystems, the need for efficient
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …

eDNA metabarcoding as a biomonitoring tool for marine protected areas

Z Gold, J Sprague, DJ Kushner, E Zerecero Marin… - PLoS …, 2021 - journals.plos.org
Monitoring of marine protected areas (MPAs) is critical for marine ecosystem management,
yet current protocols rely on SCUBA-based visual surveys that are costly and time …

Improving the reliability of eDNA data interpretation

A Burian, Q Mauvisseau, M Bulling… - Molecular Ecology …, 2021 - Wiley Online Library
Global declines in biodiversity highlight the need to effectively monitor the density and
distribution of threatened species. In recent years, molecular survey methods detecting DNA …

Integrating eDNA metabarcoding and simultaneous underwater visual surveys to describe complex fish communities in a marine biodiversity hotspot

T Valdivia‐Carrillo, A Rocha‐Olivares… - Molecular Ecology …, 2021 - Wiley Online Library
Marine biodiversity can be surveyed using underwater visual censuses and recently with
eDNA metabarcoding. Although a promising tool, eDNA studies have shown contrasting …

Uncovering the complete biodiversity structure in spatial networks: the example of riverine systems

F Altermatt, CJ Little, E Mächler, S Wang, X Zhang… - Oikos, 2020 - Wiley Online Library
Uncovering biodiversity as an inherent feature of ecosystems and understanding its effects
on ecosystem processes is one of the most central goals of ecology. Studying organisms' …

Extracting abundance information from DNA‐based data

M Luo, Y Ji, D Warton, DW Yu - Molecular Ecology Resources, 2023 - Wiley Online Library
The accurate extraction of species‐abundance information from DNA‐based data
(metabarcoding, metagenomics) could contribute usefully to diet analysis and food‐web …