Accurate diagnosis of human diseases is crucial for effective prevention, clinical treatment,
and prognosis. The World Health Organization (WHO) has provided guidelines for molecular …

Production of food-related products using microorganisms in an environmentally friendly
manner is a crucial solution to global food safety and environmental pollution issues …

Spinal muscular atrophy (SMA) is caused by mutations in SMN1. SMN2 is a paralogous
gene with a C• G-to-T• A transition in exon 7, which causes this exon to be skipped in most …

The clinical success of CRISPR therapies hinges on the safety and efficacy of Cas proteins.
The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for …

Genomic DNA of the cyanophage S-2L virus is composed of 2-aminoadenine (Z), thymine
(T), guanine (G), and cytosine (C), forming the genetic alphabet ZTGC, which violates …

X-linked chronic granulomatous disease (X-CGD) is an inborn error of immunity (IEI)
resulting from genetic mutations in the cytochrome b-245 beta chain (CYBB) gene. The …

Abstract “Test‐and‐go” single‐nucleotide variation (SNV) detection within several minutes
remains challenging, especially in low‐abundance samples, since existing methods face a …

Spinal muscular atrophy (SMA) is a devastating neuromuscular disease caused by
mutations in the SMN1 gene. Despite the development of various therapies, outcomes can …

CRISPR-Cas9 is a powerful tool for genome editing, but the strict requirement for an NGG
protospacer-adjacent motif (PAM) sequence immediately next to the DNA target limits the …

Probiotics are the treasure of the microbiology fields. They have been widely used in the
food industry, clinical treatment, and other fields. The equivocal health-promoting effects and …