Computational approaches and challenges in spatial transcriptomics

S Fang, B Chen, Y Zhang, H Sun, L Liu… - Genomics …, 2023 - academic.oup.com
The development of spatial transcriptomics (ST) technologies has transformed genetic
research from a single-cell data level to a two-dimensional spatial coordinate system and …

[HTML][HTML] Temporal progress of gene expression analysis with RNA-Seq data: A review on the relationship between computational methods

J Costa-Silva, DS Domingues, D Menotti… - Computational and …, 2023 - Elsevier
Abstract Analysis of differential gene expression from RNA-seq data has become a standard
for several research areas. The steps for the computational analysis include many data …

Translocation of viable gut microbiota to mesenteric adipose drives formation of creeping fat in humans

CWY Ha, A Martin, GD Sepich-Poore, B Shi, Y Wang… - Cell, 2020 - cell.com
A mysterious feature of Crohn's disease (CD) is the extra-intestinal manifestation of"
creeping fat"(CrF), defined as expansion of mesenteric adipose tissue around the inflamed …

Single-cell RNA sequencing reveals evolution of immune landscape during glioblastoma progression

AT Yeo, S Rawal, B Delcuze, A Christofides… - Nature …, 2022 - nature.com
Glioblastoma (GBM) is an incurable primary malignant brain cancer hallmarked with a
substantial protumorigenic immune component. Knowledge of the GBM immune …

Machine learning identifies candidates for drug repurposing in Alzheimer's disease

S Rodriguez, C Hug, P Todorov, N Moret… - Nature …, 2021 - nature.com
Clinical trials of novel therapeutics for Alzheimer's Disease (AD) have consumed a large
amount of time and resources with largely negative results. Repurposing drugs already …

Salmon provides fast and bias-aware quantification of transcript expression

R Patro, G Duggal, MI Love, RA Irizarry, C Kingsford - Nature methods, 2017 - nature.com
We introduce Salmon, a lightweight method for quantifying transcript abundance from RNA–
seq reads. Salmon combines a new dual-phase parallel inference algorithm and feature-rich …

Scaling read aligners to hundreds of threads on general-purpose processors

B Langmead, C Wilks, V Antonescu, R Charles - Bioinformatics, 2019 - academic.oup.com
Motivation General-purpose processors can now contain many dozens of processor cores
and support hundreds of simultaneous threads of execution. To make best use of these …

Power analysis of single-cell RNA-sequencing experiments

V Svensson, KN Natarajan, LH Ly, RJ Miragaia… - Nature …, 2017 - nature.com
Single-cell RNA sequencing (scRNA-seq) has become an established and powerful method
to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and …

Gaining comprehensive biological insight into the transcriptome by performing a broad-spectrum RNA-seq analysis

SME Sahraeian, M Mohiyuddin, R Sebra… - Nature …, 2017 - nature.com
Abstract RNA-sequencing (RNA-seq) is an essential technique for transcriptome studies,
hundreds of analysis tools have been developed since it was debuted. Although recent …

KEAP1/NFE2L2 Mutations Predict Lung Cancer Radiation Resistance That Can Be Targeted by Glutaminase Inhibition

MS Binkley, YJ Jeon, M Nesselbush, EJ Moding… - Cancer discovery, 2020 - AACR
Tumor genotyping is not routinely performed in localized non–small cell lung cancer
(NSCLC) due to lack of associations of mutations with outcome. Here, we analyze 232 …