Over the last years, the biopharmaceutical industry has significantly turned its biologics
production towards mammalian cell expression systems. The presence of glycosylation …

Here, we review the current state of the art in analytical and structural methods used to
characterize therapeutic monoclonal antibodies (mAbs) and derivatives. First, a general …

For over three decades, Chinese hamster ovary (CHO) cells have been the chosen
expression platform for the production of therapeutic proteins with complex post …

The present review gives an introduction into the concept of metabolomics and provides an
overview of the analytical tools applied in non-targeted metabolomics with a focus on liquid …

Metabolomics represents the global assessment of metabolites in a biological sample and
reports the closest information to the phenotype of the biological system under study …

The N-linked glycan profiles on recombinant monoclonal antibody therapeutics significantly
affect antibody biological functions and are largely determined by host cell genotypes and …

Chinese hamster ovary (CHO) cells are the primary factories for biopharmaceuticals
because of their capacity to correctly fold and post-translationally modify recombinant …

Amino acids are crucial for the cultivation of mammalian cells. This importance of amino
acids was realized soon after the development of the first cell lines, and a solution of a …

CHO cells are the most prevalent platform for modern bio‐therapeutic production. Currently,
there are several CHO cell lines used in bioproduction with distinct characteristics and …

Large scale biopharmaceutical production of biologics relies on the overexpression of
foreign proteins by cells cultivated in stirred tank bioreactors. It is well recognized and …