Modern analytical ultracentrifugation in protein science: a tutorial review

J Lebowitz, MS Lewis, P Schuck - Protein science, 2002 - Wiley Online Library
Analytical ultracentrifugation (AU) is reemerging as a versatile tool for the study of proteins.
Monitoring the sedimentation of macromolecules in the centrifugal field allows their …

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium

JL Cole, JW Lary, TP Moody, TM Laue - Methods in cell biology, 2008 - Elsevier
Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative
analysis of macromolecules in solution. AUC has broad applications for the study of …

Size-distribution analysis of macromolecules by sedimentation velocity ultracentrifugation and lamm equation modeling

P Schuck - Biophysical journal, 2000 - cell.com
A new method for the size-distribution analysis of polymers by sedimentation velocity
analytical ultracentrifugation is described. It exploits the ability of Lamm equation modeling …

[HTML][HTML] Size-distribution analysis of proteins by analytical ultracentrifugation: strategies and application to model systems

P Schuck, MA Perugini, NR Gonzales, GJ Howlett… - Biophysical journal, 2002 - cell.com
Strategies for the deconvolution of diffusion in the determination of size-distributions from
sedimentation velocity experiments were examined and developed. On the basis of four …

On the analysis of protein self-association by sedimentation velocity analytical ultracentrifugation

P Schuck - Analytical biochemistry, 2003 - Elsevier
Analytical ultracentrifugation is one of the classical techniques for the study of protein
interactions and protein self-association. Recent instrumental and computational …

Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation

PH Brown, P Schuck - Biophysical journal, 2006 - cell.com
Sedimentation velocity analytical ultracentrifugation is an important tool in the
characterization of macromolecules and nanoparticles in solution. The sedimentation …

Determination of nanoparticle size distribution together with density or molecular weight by 2D analytical ultracentrifugation

RP Carney, JY Kim, H Qian, R Jin, H Mehenni… - Nature …, 2011 - nature.com
Nanoparticles are finding many research and industrial applications, yet their
characterization remains a challenge. Their cores are often polydisperse and coated by a …

Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation

H Zhao, CA Brautigam, R Ghirlando… - Current protocols in …, 2013 - Wiley Online Library
Modern computational strategies have allowed for the direct modeling of the sedimentation
process of heterogeneous mixtures, resulting in sedimentation velocity (SV) size‐distribution …

Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition

J Vistica, J Dam, A Balbo, E Yikilmaz, RA Mariuzza… - Analytical …, 2004 - Elsevier
Sedimentation equilibrium is a powerful tool for the characterization of protein self-
association and heterogeneous protein interactions. Frequently, it is applied in a …

Determination of the sedimentation coefficient distribution by least‐squares boundary modeling

P Schuck, P Rossmanith - Biopolymers: Original Research on …, 2000 - Wiley Online Library
A new method is presented for the calculation of apparent sedimentation coefficient
distributions g*(s) for the size‐distribution analysis of polymers in sedimentation velocity …