Concerns over climate change have necessitated a rethinking of our transportation
infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced …

Reconstructed microbial metabolic networks facilitate a mechanistic description of the
genotype–phenotype relationship through the deployment of constraint-based …

Network reconstructions are a common denominator in systems biology. Bottom–up
metabolic network reconstructions have been developed over the last 10 years. These …

The field of metabolic engineering is primarily concerned with improving the biological
production of value-added chemicals, fuels and pharmaceuticals through the design …

After hundreds of generations of adaptive evolution at exponential growth, Escherichia coli
grows as predicted using flux balance analysis (FBA) on genome‐scale metabolic models …

Although the network topology of metabolism is well known, understanding the principles
that govern the distribution of fluxes through metabolism lags behind. Experimentally, these …

Abstract Systems analysis of metabolic and growth functions in microbial organisms is
rapidly developing and maturing. Such studies are enabled by reconstruction, at the …

The genome‐scale model (GEM) of metabolism in the bacterium Escherichia coli K‐12 has
been in development for over a decade and is now in wide use. GEM‐enabled studies of E …

The combination of synthetic and systems biology is a powerful framework to study
fundamental questions in biology and produce chemicals of immediate practical application …

To which extent can optimality principles describe the operation of metabolic networks? By
explicitly considering experimental errors and in silico alternate optima in flux balance …