The last decade has seen a robust increase in various types of novel RNA molecules and
their complexity in gene regulation. RNA molecules play a critical role in cellular events by …

Targeting RNAs appears as an important opportunity to modulate biological processes.
Here, we overviewed critical parameters implied in RNAs competition to bind small RNAs …

We describe the synthesis and RNA acylation activity of a series of minimalist azidoalkanoyl
imidazole reagents, with the aim of functionalizing RNA at 2′-hydroxyl groups at …

The genes encoding Hfq-dependent sRNAs possess a typical Rho-independent
transcription terminator. Here, we have studied the molecular events occurring at Rho …

Labeled RNAs are invaluable probes for investigation of RNA function and localization.
However, mRNA labeling remains challenging. Here, we developed an improved method for …

Regulatory RNA-based interactions are critical for coordinating gene expression and are
increasingly being targeted in synthetic biology, antimicrobial, and therapeutic fields …

We are just beginning to unravel the myriad of interactions in which non-coding RNAs
participate. The intricate RNA interactome is the foundation of many biological processes …

Non-coding regulatory RNAs (ncRNAs) comprise specialized group of essential genetically
encoded biological molecules involved in the wide variety of cellular metabolic processes …

Anotace Tato práce se zabývá interakcí thymidylát syntázy (TYMS) se svým vlastním
transkriptem. Za tímto účelem byl vytvořen nový přístup pro charakterizaci interakce RNA …

In this thesis, different methods were used to analyse thymidylate synthase binding to its
transcript. To this end, a novel approach is described that characterises the RNA-protein …